Amos is from Marobela Village in the North Eastern part of Botswana. He went to Shangano Community Junior Secondary School (2007–2009) in Nshakazhogwe and Tutume McConnel Community College (2010–2012).
After an injury filled 2013 season, Amos returned to form in 2014. At the 2014 Prefontaine Classic, Amos set a meet record and world leading time of 1:43.63.  At the HerculisIAAF Diamond League, he again set a meet record and world leading mark of 1:42.45.  Beating Rudisha for the 2nd time on the season, his performance was the fastest 800m race since the men's 800 m race. At the 2014 Commonwealth Games, Amos won the 800 meter Gold medal in 1:45.18. In the tactical affair, Amos maneuvered out of a box to pass World Record holder David Rudisha in the last 50 meters.