Nile blue
From Wikipedia, the free encyclopedia
| Nile blue | |
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| IUPAC name |
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| Other names | Nile blue A |
| Identifiers | |
| CAS number | [] |
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| Properties | |
| Molecular formula | C20H20ClN3O |
| Molar mass | 353.845 g/mol |
| Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa) Infobox references |
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Nile blue (or Nile blue A) is a stain used in biology and histology. It may be used with live or fixed cells, and imparts a blue colour to cell nuclei.
It may also be used in conjunction with fluorescence microscopy to stain for the presence of polyhydroxybutyrate granules in prokaryotic or eukaryotic cells. Boiling a solution of Nile blue with sulfuric acid produces Nile red (Nile blue oxazone).
