Olfactory receptors expressed in the cell membranes of olfactory receptor neurons are responsible for the detection of odor molecules. Activated olfactory receptors are the initial player in a signal transduction cascade which ultimately produces a nerve impulse which is transmitted to the brain. These receptors are members of the class A rhodopsin-like family of G protein-coupled receptors (GPCRs). The olfactory receptors form a multigene family consisting of over 900 genes in humans and 1500 genes in mice.
In vertebrates, the olfactory receptors are located in both the cilia and synapses of the olfactory sensory neurons and in the epithelium of the human airway. In insects, olfactory receptors are located on the antennae and other chemosensory organs. Sperm cells also express odor receptors, which are thought to be involved in chemotaxis to find the egg cell.
Rather than binding specific ligands, olfactory receptors display affinity for a range of odor molecules, and conversely a single odorant molecule may bind to a number of olfactory receptors with varying affinities, which depend on physio-chemical properties of molecules like their molecular volumes . Once the odorant has bound to the odor receptor, the receptor undergoes structural changes and it binds and activates the olfactory-type G protein on the inside of the olfactory receptor neuron. The G protein (Golf and/or Gs) in turn activates the lyase - adenylate cyclase - which converts ATP into cyclic AMP (cAMP). The cAMP opens cyclic nucleotide-gated ion channels which allow calcium and sodium ions to enter into the cell, depolarizing the olfactory receptor neuron and beginning an action potential which carries the information to the brain.
The primary sequences of thousands of olfactory receptors (ORs) are known from the genomes of more than a dozen organisms: they are seven-helix transmembrane proteins, but there are (as of July 2011) no known structures of any OR. There is a highly conserved sequence in roughly three quarters of all ORs that is a tripodal metal ion binding site, and Suslick has proposed that the ORs are in fact metalloproteins (mostly likely with zinc, copper and possibly manganese ions) that serve as a Lewis acid site for binding of many odorant molecules. Crabtree, in 1978, had previously suggested that Cu(I) is "the most likely candidate for a metallo-receptor site in olfaction" for strong-smelling volatiles which are also good metal-coordinating ligands, such as thiols. Zhuang, Matsunami and Block, in 2012, confirmed the Crabtree/Suslick proposal for the specific case of a mouse OR, MOR244-3, showing that copper is essential for detection of certain thiols and other sulfur-containing compounds. Thus, by using a chemical that binds to copper in the mouse nose, so that copper wasn’t available to the receptors, the authors showed that the mice couldn't detect the thiols. However, these authors also found that MOR244-3 lacks the specific metal ion binding site suggested by Suslick, instead showing a different motif in the EC2 domain.
In a recent but highly controversial interpretation, it has also been speculated that olfactory receptors might really sense various vibrational energy-levels of a molecule rather than structural motifs via quantum coherence mechanisms. As evidence it has been shown that flies can differentiate between two odor molecules which only differ in hydrogen isotope (which will drastically change vibrational energy levels of the molecule). Not only could the flies distinguish between the deuterated and non-deuterated forms of an odorant, they could generalise the property of "deuteratedness" to other novel molecules. In addition, they generalised the learned avoidance behaviour to molecules which were not deuterated but did share a significant vibration stretch with the deuterated molecules, a fact which the differential physics of deuteration (below) has difficulty in accounting for.
It should be noted, however, that deuteration changes the heats of adsorption and the boiling and freezing points of molecules (boiling points: 100.0 °C for H2O vs. 101.42 °C for D2O; melting points: 0.0 °C for H2O, 3.82 °C for D2O), pKa (i.e., dissociation constant: 9.71x10−15 for H20 vs. 1.95x10−15 for D2O, cf. heavy water) and the strength of hydrogen bonding. Such isotope effects are exceedingly common, and so it is well known that deuterium substitution will indeed change the binding constants of molecules to protein receptors.
There are a large number of different odor receptors, with as many as 1,000 in the mammalian genome which represents approximately 3% of the genes in the genome. However not all of these potential odor receptor genes are expressed and functional. According to an analysis of data derived from the human genome project, humans have approximately 400 functional genes coding for olfactory receptors and the remaining 600 candidates are pseudogenes.
The reason for the large number of different odor receptors is to provide a system for discriminating between as many different odors as possible. Even so, each odor receptor does not detect a single odor. Rather each individual odor receptor is broadly tuned to be activated by a number of similar odorant structures. Analogous to the immune system, the diversity that exists within the olfactory receptor family allows molecules that have never been encountered before to be characterized. However, unlike the immune system, which generates diversity through in-situ recombination, every single olfactory receptor is translated from a specific gene; hence the large portion of the genome devoted to encoding OR genes. Furthermore most odors activate more than one type of odor receptor. Since the number of combinations and permutations of olfactory receptors is almost limitless, the olfactory receptor system is capable of detecting and distinguishing between a practically infinite number of odorant molecules.
A nomenclature system has been devised for the olfactory receptor family and is the basis for the official Human Genome Project (HUGO) symbols for the genes that encode these receptors. The names of individual olfactory receptor family members are in the format "ORnXm" where:
- OR is the root name (Olfactory Receptor superfamily)
- n = an integer representing a family (e.g., 1-56) whose members have greater than 40% sequence identity,
- X = a single letter (A, B, C, ...) denoting a subfamily (>60% sequence identity), and
- m = an integer representing an individual family member (isoform).
For example OR1A1 is the first isoform of subfamily A of olfactory receptor family 1.
Members belonging to the same subfamily of olfactory receptors (>60% sequence identity) are likely to recognize structurally similar odorant molecules.
Two major classes of olfactory receptors have been identified in humans:
- class I (fish-like receptors) OR families 51-56
- class II (tetrapod specific receptors) OR families 1-13
The olfactory receptor gene family in vertebrates has been shown to evolve through genomic events such as gene duplication or gene conversion. Evidence of a role for tandem duplication is provided by the fact that many olfactory receptor genes belonging to the same phylogenetic clade are located in the same gene cluster. To this point, the organization of OR genomic clusters is well conserved between humans and mice, even though the functional OR count is vastly different between these two species. Such birth-and-death evolution has brought together segments from several OR genes to generate and degenerate odorant binding site configurations, creating new functional OR genes as well as pseudogenes.
Compared to many other mammals, primates have a relatively small number of functional OR genes. For instance, since divergence from their most recent common ancestor (MRCA), mice have gained a total of 623 new OR genes, and lost 285 genes, whereas humans have gained only 83 genes, but lost 428 genes. Mice have a total of 1035 OR genes, humans have 387 OR genes. The vision priority hypothesis states that the evolution of color vision in primates may have decreased primate reliance on olfaction, which explains the relaxation of selective pressure that accounts for the accumulation of olfactory receptor pseudogenes in primates. However, recent evidence has rendered the vision priority hypothesis obsolete, because it was based on misleading data and assumptions. The hypothesis assumed that functional OR genes can be correlated to the olfactory capability of a given animal. In this view, a decrease in the fraction of functional OR genes would cause a reduction in the sense of smell; species with higher pseudogene count would also have a decreased olfactory ability. This assumption is flawed. Dogs, which are reputed to have good sense of smell, do not have the largest number of functional OR genes. Additionally, pseudogenes may be functional; 67% of human OR pseudogenes are expressed in the main olfactory epithelium, where they possibly have regulatory roles in gene expression. More importantly, the vision priority hypothesis assumed a drastic loss of functional OR genes at the branch of the OWMs, but this conclusion was biased by low-resolution data from only 100 OR genes. High-resolution studies instead agree that primates have lost OR genes in every branch from the MRCA to humans, indicating that the degeneration of OR gene repertories in primates cannot simply be explained by the changing capabilities in vision.
It has been shown that negative selection is still relaxed in modern human olfactory receptors, suggesting that no plateau of minimal function has yet been reached in modern humans and therefore that olfactory capability might still be decreasing. This is considered to provide a first clue to the future human genetic evolution.
In 2004 Linda B. Buck and Richard Axel won the Nobel Prize in Physiology or Medicine for their work on olfactory receptors. In 2006 it was shown that another class of odorant receptors exist for volatile amines. This class of receptors consists of the trace amine-associated receptors (TAAR), including the primary biomolecular target of amphetamine and its endogenous analogues, TAAR1. 3-Iodothyronamine, a thyroid hormone, is also known to activate the receptor.
- Gaillard I, Rouquier S, Giorgi D (2004). "Olfactory receptors". Cell. Mol. Life Sci. 61 (4): 456–69. doi:10.1007/s00018-003-3273-7. PMID 14999405.
- Hussain A, Saraiva LR, Korsching SI (2009). "Positive Darwinian selection and the birth of an olfactory receptor clade in teleosts". PNAS 106 (11): 4313–8. Bibcode:2009PNAS..106.4313H. doi:10.1073/pnas.0803229106. PMC 2657432. PMID 19237578.
- Niimura Y and Nei M (2003). "Evolution of olfactory receptor genes in the human genome". PNAS 100 (21): 12235–40. Bibcode:2003PNAS..10012235N. doi:10.1073/pnas.1635157100. PMC 218742. PMID 14507991.
- Rinaldi A (2007). "The scent of life. The exquisite complexity of the sense of smell in animals and humans". EMBO Reports 8 (7): 629–33. doi:10.1038/sj.embor.7401029. PMC 1905909. PMID 17603536.
- Gu X, Karp PH, Brody SL, Pierce, RA, Welsh MJ, Holtzman MJ, Ben-Shahar Y (2013). "Volatile-sensing functions for pulmonary neuroendocrine cells". American journal of respiratory cell and molecular biology 50 (3): 637–46. doi:10.1165/rcmb.2013-0199OC. PMID 24134460.
- Hallem EA, Dahanukar A, Carlson JR (2006). "Insect odor and taste receptors". Annu. Rev. Entomol. 51: 113–35. doi:10.1146/annurev.ento.51.051705.113646. PMID 16332206.
- Spehr M, Schwane K, Riffell JA, Zimmer RK, Hatt H (2006). "Odorant receptors and olfactory-like signaling mechanisms in mammalian sperm". Mol. Cell. Endocrinol. 250 (1–2): 128–36. doi:10.1016/j.mce.2005.12.035. PMID 16413109.
- Buck LB (2004). "Olfactory receptors and odor coding in mammals". Nutr. Rev. 62 (11 Pt 2): S184–8; discussion S224–41. doi:10.1301/nr.2004.nov.S184-S188. PMID 15630933.
- Saberi M, Seyed-allaei (2015). "Olfactory receptors are sensitive to molecular volume of odorants". bioRxiv. doi:10.1101/013516.
- Jones DT, Reed RR (1989). "Golf: an olfactory neuron specific-G protein involved in odorant signal transduction". Science 244 (4906): 790–5. Bibcode:1989Sci...244..790J. doi:10.1126/science.2499043. PMID 2499043.
- Wang J., Luthey-Schulten Z., Suslick K. S. (2003). "Is the Olfactory Receptor A Metalloprotein?". Proc. Natl. Acad. Sci. U.S.A 100 (6): 3035–3039. Bibcode:2003PNAS..100.3035W. doi:10.1073/pnas.262792899. PMC 152240. PMID 12610211.
- Crabtree RH (1978). "Copper (I): A possible olfactory binding site". Journal of Inorganic and Nuclear Chemistry 40 (7): 1453. doi:10.1016/0022-1902(78)80071-2.
- Duan X, Block E, Li Z, Connelly T, Zhang J, Huang Z, Su X, Pan Y, Wu L, Chi Q, Thomas S, Zhang S, Ma M, Matsunami H, Chen GQ, Zhuang H (February 2012). "Crucial role of copper in detection of metal-coordinating odorants". Proc. Natl. Acad. Sci. U.S.A. 109 (9): 3492–7. Bibcode:2012PNAS..109.3492D. doi:10.1073/pnas.1111297109. PMC 3295281. PMID 22328155.
- Brookes JC, Hartoutsiou F, Horsfield AP, Stoneham AM (January 2007). "Could humans recognize odor by phonon assisted tunneling?". Phys. Rev. Lett. 98 (3): 038101. arXiv:physics/0611205. Bibcode:2007PhRvL..98c8101B. doi:10.1103/PhysRevLett.98.038101. PMID 17358733.
- Franco MI, Turin L, Mershin A, Skoulakis EM. (2011). "Molecular vibration-sensing component in Drosophila melanogaster olfaction". PNAS 108 (9): 3797–802. Bibcode:2011PNAS..108.3797F. doi:10.1073/pnas.1012293108. PMC 3048096. PMID 21321219.
- Schramm VL (October 2007). "Binding isotope effects: boon and bane". Curr Opin Chem Biol 11 (5): 529–36. doi:10.1016/j.cbpa.2007.07.013. PMC 2066183. PMID 17869163.
- Gilad Y, Lancet D (2003). "Population differences in the human functional olfactory repertoire". Mol. Biol. Evol. 20 (3): 307–14. doi:10.1093/molbev/msg013. PMID 12644552.
- Malnic B, Hirono J, Sato T, Buck LB (1999). "Combinatorial receptor codes for odors". Cell 96 (5): 713–23. doi:10.1016/S0092-8674(00)80581-4. PMID 10089886.
- Araneda RC, Peterlin Z, Zhang X, Chesler A, Firestein S (2004). "A pharmacological profile of the aldehyde receptor repertoire in rat olfactory epithelium". J. Physiol. (Lond.) 555 (Pt 3): 743–56. doi:10.1113/jphysiol.2003.058040. PMC 1664868. PMID 14724183.
- Glusman G, Bahar A, Sharon D, Pilpel Y, White J, Lancet D (2000). "The olfactory receptor gene superfamily: data mining, classification, and nomenclature". Mamm. Genome 11 (11): 1016–23. doi:10.1007/s003350010196. PMID 11063259.
- Malnic B, Godfrey PA, Buck LB (2004). "The human olfactory receptor gene family". PNAS 101 (8): 2584–9. Bibcode:2004PNAS..101.2584M. doi:10.1073/pnas.0307882100. PMC 356993. PMID 14983052.
- Glusman G, Yanai I, Rubin I, Lancet D (2001). "The complete human olfactory subgenome". Genome Res. 11 (5): 685–702. doi:10.1101/gr.171001. PMID 11337468.
- Nei M and Rooney AP (2005). "Concerted and birth-and-death evolution of multigene families". Annu Rev Genet. 39: 121–152. doi:10.1146/annurev.genet.39.073003.112240. PMC 1464479. PMID 16285855.
- Niimura Y and Nei M (2006). "Evolutionary dynamics of olfactory and other chemosensory receptor genes in vertebrates". Journal of Human Genetics 51 (6): 505–517. doi:10.1007/s10038-006-0391-8. PMC 1850483. PMID 16607462.
- Niimura Y and Nei M (2005). "Comparative evolutionary analysis of olfactory receptor gene clusters between humans and mice". Gene 346 (6): 13–21. doi:10.1016/j.gene.2004.09.025. PMID 15716120.
- Nozawa M, Nei M (2008). "Genomic drift and copy number variation of chemosensory receptor genes in humans and mice". Cytogenet. Genome Res. 123 (1-4): 263–9. doi:10.1159/000184716. PMC 2920191. PMID 19287163.
- Niimura Y, Nei M (2007). "Extensive gains and losses of olfactory receptor genes in mammalian evolution". PLoS ONE 2 (8): e708. doi:10.1371/journal.pone.0000708. PMC 1933591. PMID 17684554.
- Gilad Y, Wiebe V, Przeworski M, Lancet D, Paabo S (2004). "Loss of Olfactory Receptor Genes Coincides with the Acquisition of Full Trichromatic Vision in Primates". PLOS Biology 2 (1): 120–125. doi:10.1371/journal.pbio.0020005. PMC 314465. PMID 14737185.
- Craven, B. A., E. G. Paterson, and G. S. Settles. (2010). "The fluid dynamics of canine olfaction: Unique nasal airflow patterns as an explanation of macrosmia". Journal of the Royal Society Interface 7 (47): 933–943. doi:10.1098/Rsif.2009.0490.
- Zhang X, De la Cruz O, Pinto JM, Nicolae D, Firestein S, Gilad Y (2007). "Characterizing the expression of the human olfactory receptor gene family using a novel DNA microarray". Genome Biol. 8 (5): R86. doi:10.1186/gb-2007-8-5-r86. PMC 1929152. PMID 17509148.
- Matsui, A., Y. Go, and Y. Niimura (2010). "Degeneration of olfactory receptor gene repertories in primates: No direct link to full trichromatic vision". Molecular Biology and Evolution 27 (5): 1192–1200. doi:10.1093/molbev/msq003. PMID 20061342.
- Niimura Y (April 2012). "Olfactory receptor multigene family in vertebrates: from the viewpoint of evolutionary genomics". Curr. Genomics 13 (2): 103–14. doi:10.2174/138920212799860706. PMC 3308321. PMID 23024602.
- Pierron D, Cortés NG, Letellier T, Grossman LI. (2013). "Current relaxation of selection on the human genome: Tolerance of deleterious mutations on olfactory receptors". Mol Phylogenet Evol.. 66 (2): 558–564. doi:10.1016/j.ympev.2012.07.032. PMID 22906809.
- Buck L, Axel R (1991). "A novel multigene family may encode odorant receptors: a molecular basis for odor recognition". Cell 65 (1): 175–87. doi:10.1016/0092-8674(91)90418-X. PMID 1840504.
- "Press Release: The 2004 Nobel Prize in Physiology or Medicine". Retrieved 2007-06-06.
- Liberles SD, Buck LB (2006). "A second class of chemosensory receptors in the olfactory epithelium". Nature 442 (7103): 645–50. Bibcode:2006Natur.442..645L. doi:10.1038/nature05066. PMID 16878137.
- Miller GM (January 2011). "The emerging role of trace amine-associated receptor 1 in the functional regulation of monoamine transporters and dopaminergic activity". J. Neurochem. 116 (2): 164–176. doi:10.1111/j.1471-4159.2010.07109.x. PMC 3005101. PMID 21073468.
- Broadley KJ (March 2010). "The vascular effects of trace amines and amphetamines". Pharmacol. Ther. 125 (3): 363–375. doi:10.1016/j.pharmthera.2009.11.005. PMID 19948186.
Fig. 2. Synthetic and metabolic pathways for endogenous and exogenously administered trace amines and sympathomimetic amines ...
Trace amines are metabolized in the mammalian body via monoamine oxidase (MAO; EC 18.104.22.168) (Berry, 2004) (Fig. 2) ... It deaminates primary and secondary amines that are free in the neuronal cytoplasm but not those bound in storage vesicles of the sympathetic neurone ...
Thus, MAO inhibitors potentiate the peripheral effects of indirectly acting sympathomimetic amines. It is not often realized, however, that this potentiation occurs irrespective of whether the amine is a substrate for MAO. An α-methyl group on the side chain, as in amphetamine and ephedrine, renders the amine immune to deamination so that they are not metabolized in the gut. Similarly, β-PEA would not be deaminated in the gut as it is a selective substrate for MAO-B which is not found in the gut ...
Brain levels of endogenous trace amines are several hundred-fold below those for the classical neurotransmitters noradrenaline, dopamine and serotonin but their rates of synthesis are equivalent to those of noradrenaline and dopamine and they have a very rapid turnover rate (Berry, 2004). Endogenous extracellular tissue levels of trace amines measured in the brain are in the low nanomolar range. These low concentrations arise because of their very short half-life ...
- Khafizov K, Anselmi C, Menini A, Carloni P (2007). "Ligand specificity of odorant receptors". J Mol Model 13 (3): 401–9. doi:10.1007/s00894-006-0160-9. PMID 17120078.
- Olfactory Receptor Database
- Human Olfactory Receptor Data Exploratorium (HORDE)
- Olfactory Receptor Protein at the US National Library of Medicine Medical Subject Headings (MeSH)