It is sung by a quintet of drunk fish in Porky's Duck Hunt in a rowboat, after they swim through a sunken whiskey barrel shot by Porky Pig (1937). It is again sung by Porky himself in the ironically titled 1942 cartoon, My Favorite Duck, in which Porky is tormented by Daffy Duck while on a camping trip. It well fits Porky's speech impediment — which is especially noticeable with M's and B's. Daffy, meanwhile, keeps singing "Blues in the Night (My Mama Done Tol' Me)". At one point, Porky unconsciously starts to sing Daffy's number, then stops, looks into the camera with a "Harumph!" and returns to his stuttering version of "Moonlight Bay.". The song was also used in another Porky cartoon, entitled "Trap Happy Porky" (1945), and it was sung by a quintet of drunk cats (one of them was playing the piano).
On Season 2, Episode 3 of Taxi, Louie DePalma (Danny DeVito) sings Moonlight Bay while under the influence of whatever "Reverend" Jim Ignatowski (Christopher Lloyd) has slipped into his coffee. It is part of an effort by the cabbies to get Louie to hire Jim at the cab company, something he has refused to do. Louie's final words, just before drifting off, are, "Everyone works on Moonlight Bay".