Highway 52 passes beneath high-tension lines north of Highway 403
Highway 52 was a rural highway on the outskirts of Hamilton. Although it has been locally maintained for a number of years, the surrounding have remained relatively unaltered since then. The route begins at an intersection with former Highway 2 and Highway 53 west of Ancaster. From there the highway travelled north, interchanging with Highway 403 at Exit 55. The route passes through the communities of Summit and Copetown, intersecting the former Highway 99, the Governors Road, in the latter. Soon thereafter, it intersects the former western leg of Highway 5. Immediately north of this, the route encounters Highway 8 at a second intersection, where it ended during the final decade of its existence.
Highway 52 originally travelled from Highway 8 in Rockton north along at least five different township roads over 13 kilometres (8 mi), ending at a local township road at the Wellington/Wentworth County Line. This section was assumed by the Department of Highways on September 1, 1937. On April 13, 1938, a dirt road from Peter's Corners south to Highway 2 and Highway 53 (Wilson Street) in Ancaster was assumed as Highway 52. This added a 7-kilometre (4 mi) multiplex with Highway 8, a full quarter of Highway 52's entire length. Throughout World War II, the new section of Highway 52 remained unimproved; in 1945 it was gravelled. The road was paved between Highway 2 and Highway 97 in 1955, with the remainder being paved three years later.
For most of its existence, Highway 52 ended inexplicably at this intersection with Gore Road, at the Hamilton – Wellington County boundary
The original section of Highway 52 north of Peters Corners was downloaded to the Regional Municipality of Hamilton–Wentworth in the mid-1980s, around the same time as the decommissioning of Highway 97. The road's length was reduced to 10.7 kilometres (6.6 mi) and the concurrency with Highway 8 removed. On April 1, 1998, the remainder of Highway 52 was downloaded to the Region of Hamilton-Wentworth, now the City of Hamilton. The road has not been given a new numerical designation, and is simply known as Westover Road and Trinity Road.