* Party did not nominate candidates in previous election.
^Leslie Oke and Beniah Bowman broke with the Progressives as they were opposed to William Raney's leadership as he was not a farmer and were also opposed to the creation of a new Progressive Party which would not exclusively be a farmers party. Bowman resigned from the legislature before the election but was succeeded in his Manitoulin riding by Thomas Farquhar. The UFO picked up a third seat, Grey South, where Farquhar Oliver won with the assistance of federal MP Agnes MacPhail. The three UFO members did not join the Progressive caucus after the election.
^The Labour candidates were not a unified bloc in this election. Labour MLAs caucused with the Progressives in the previous legislature and were often referred to as Progressive-Labour MLAs. They were not united behind Progressive leader Raney over the main issue of the campaign, prohibition, with Karl Homuth, the sole Labour MLA who was re-elected in 1926, supporting the Conservative government's policy in favour of allowing the sale of liquor. Another Labour candidate, H.A. Stevenson in London South ran as a Labor-Prohibitionist while a fourth, A.E. Smith in Hamilton Centre was the candidate of the Canadian Labour Party and a member of the Communist Party of Canada.
^Karl Homuth is the sole Labour MLA elected. He ran for re-election on a platform supporting Premier Ferguson's policy on repealing the Ontario Temperance Act and supported the government in the legislature. Joins the Conservatives in the next election.