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From 1872 to 1876 Perutz was director of Bayerische Aktiengesellschaft für chemische und landwirtschaftlich-chemische Fabrikate (Bavarian Corporation for Chemical and Agrochemical Products Inc., later Süd-Chemie AG) in Munich-Heufeld.
Film box of the color film Perutz C 18 (18 DIN)
In 1880 he founded his own firm Otto Perutz Trockenplattenfabrik in Munich. He developed a method for the industrial production of Eosin-Silver-Plates which had been invented by Hermann Wilhelm Vogel and Johann Baptist Obernetter. This was crucial to develop colour photography. In 1896 Perutz-Plates were used for radiography for the first time.
Perutz sold his firm in 1897. He was member of the supervisory board of Bayerische Aktiengesellschaft für chemische und landwirtschaftlich-chemische Fabrikate from 1902 until his death in 1922. The Perutz-Photowerke became part of Agfa in 1964.