|PA clan of proteases|
The PA clan (Proteases of mixed nucleophile, superfamily A) is the largest group of proteases with common ancestry as identified by structural homology. Members have a chymotrypsin-like fold and similar proteolysis mechanisms but sequence identity of <10%. The clan contains both cysteine and serine proteases (different nucleophiles). PA clan proteases can be found in plants, animals, fungi, eubacteria, archaea and viruses.
In the 1960s, the sequence similarity of several proteases indicated that they were evolutionarily related. These were grouped into the chymotrypsin-like serine proteases (now called the S1 family). As the structures of these, and other proteases were solved by X-ray crystallography in the 1970s and 80s, it was noticed that several viral proteases such as Tobacco Etch Virus protease showed structural homology despite no discernible sequence similarity and even a different nucleophile. Based on structural homology, a superfamily was defined and later named the PA clan (by the MEROPS classification system). As more structures are solved, more protease families have been added to the PA clan superfamily.
The P refers to Proteases of mixed nucleophile. The A indicates that it was the first such clan to be identified (there also exist the PB, PC, PD and PE clans).
Despite retaining as little as 10% sequence identity, PA clan members isolated from viruses, prokaryotes and eukaryotes show structural homology and can be aligned by structural similarity (e.g. with DALI).
PA clan proteases all share a core motif of two β-barrels with covalent catalysis performed by an acid-histidine-nucleophile catalytic triad motif. The barrels are arranged perpendicularly beside each other with hydrophobic residues holding them together as the core scaffold for the enzyme. The triad residues are split between the two barrels so that catalysis takes place at their interface.
Viral protease loop
In addition to the double β-barrel core, some viral proteases (such as TEV protease have a long, flexible C-terminal loop that forms a lid to which completely covers the substrate and create a binding tunnel. This tunnel contains a set of tight binding pockets such that each side chain of the substrate peptide (P6 to P1’) is bound in a complementary site (S6 to S1’) and specificity is endowed by the large contact area between enzyme and substrate. Conversely, cellular proteases that lack this loop, such as trypsin have broader specificity.
Evolution and function
The structural homology described above indicates that the PA clan members are descended from a common ancestor of the same fold. Although PA clan proteases share a common ancestor some families use serine as the nucleophile in their catalytic triad whilst others use cysteine. The catalytic triad is a set of three residues that together perform nucelophilic catalysis and are therefore a vital component of the enzyme active site. This is therefore an extreme example of divergent enzyme evolution since during evolutionary history, the core catalytic residue of the enzyme has switched. All cellular PA clan proteases are serine proteases, however there are both serine and cysteine protease families of viral proteases.
In addition to divergence in their core catalytic machinery, the PA clan proteases also show wide divergent evolution in function. Members of the PA clan can be found in eukaryotes, prokaryotes and viruses and encompass a wide range of functions.
In mammals, some are involved in blood clotting (e.g. thrombin) and so have high substrate specificity as well as digestion (e.g. trypsin) with broad substrate specificity. Several snake venoms are also PA clan proteases, such as pit viper haemotoxin and interfere with the victim's blood clotting cascade. Additionally, bacteria such as Staphylococcus aureus secrete exfoliative toxin which digest and damage the host's tissues. Finally, many viruses express their genome as a single, massive polyprotein and use a protease to cleave this into functional units (e.g. polio, norovirus, and TEV proteases).
Within the PA clan (P=proteases of mixed nucleophiles), families are designated by their catalytic nucleophile (C=cysteine proteases, S=serine proteases). Despite the lack of sequence homology for the PA clan as a whole, individual families within it can be identified by sequence similarity.
- Rawlings, ND; Barrett, AJ; Bateman, A (January 2012). "MEROPS: the database of proteolytic enzymes, their substrates and inhibitors.". Nucleic Acids Research 40 (Database issue): D343–50. doi:10.1093/nar/gkr987. PMC 3245014. PMID 22086950.
- Bazan, JF; Fletterick, RJ (November 1988). "Viral cysteine proteases are homologous to the trypsin-like family of serine proteases: structural and functional implications.". Proceedings of the National Academy of Sciences of the United States of America 85 (21): 7872–6. doi:10.1073/pnas.85.21.7872. PMC 282299. PMID 3186696.
- Laskar, A; Rodger, EJ; Chatterjee, A; Mandal, C (May 24, 2012). "Modeling and structural analysis of PA clan serine proteases.". BMC research notes 5: 256. doi:10.1186/1756-0500-5-256. PMID 22624962.
- Barbosa, JA; Saldanha, JW; Garratt, RC (July 1996). "Novel features of serine protease active sites and specificity pockets: sequence analysis and modelling studies of glutamate-specific endopeptidases and epidermolytic toxins.". Protein engineering 9 (7): 591–601. doi:10.1093/protein/9.7.591. PMID 8844831.
- "MEROPS - Archeal S01 proteases". Retrieved 2013.
- Ruiz-Perez, F; Nataro, JP (May 21, 2013). "Bacterial serine proteases secreted by the autotransporter pathway: classification, specificity, and role in virulence.". Cellular and molecular life sciences : CMLS 71 (5): 745–70. doi:10.1007/s00018-013-1355-8. PMID 23689588.
- Buller, AR; Townsend, CA (Feb 19, 2013). "Intrinsic evolutionary constraints on protease structure, enzyme acylation, and the identity of the catalytic triad.". Proceedings of the National Academy of Sciences of the United States of America 110 (8): E653–61. doi:10.1073/pnas.1221050110. PMID 23382230.
- de Haën, C; Neurath, H; Teller, DC (Feb 25, 1975). "The phylogeny of trypsin-related serine proteases and their zymogens. New methods for the investigation of distant evolutionary relationships.". Journal of Molecular Biology 92 (2): 225–59. doi:10.1016/0022-2836(75)90225-9. PMID 1142424.
- Lesk, AM; Fordham, WD (May 10, 1996). "Conservation and variability in the structures of serine proteinases of the chymotrypsin family.". Journal of Molecular Biology 258 (3): 501–37. doi:10.1006/jmbi.1996.0264. PMID 8642605.
- Gorbalenya, AE; Blinov, VM; Donchenko, AP (Jan 6, 1986). "Poliovirus-encoded proteinase 3C: a possible evolutionary link between cellular serine and cysteine proteinase families.". FEBS Letters 194 (2): 253–7. doi:10.1016/0014-5793(86)80095-3 (inactive 2014-09-30). PMID 3000829.
- Phan, J; Zdanov, A; Evdokimov, AG; Tropea, JE; Peters HK, 3rd; Kapust, RB; Li, M; Wlodawer, A; Waugh, DS (Dec 27, 2002). "Structural basis for the substrate specificity of tobacco etch virus protease.". The Journal of Biological Chemistry 277 (52): 50564–72. doi:10.1074/jbc.M207224200. PMID 12377789.
- Allaire, M; Chernaia, MM; Malcolm, BA; James, MN (May 5, 1994). "Picornaviral 3C cysteine proteinases have a fold similar to chymotrypsin-like serine proteinases.". Nature 369 (6475): 72–6. doi:10.1038/369072a0. PMID 8164744.
- Snijder, EJ; Wassenaar, AL; van Dinten, LC; Spaan, WJ; Gorbalenya, AE (Mar 1, 1996). "The arterivirus nsp4 protease is the prototype of a novel group of chymotrypsin-like enzymes, the 3C-like serine proteases.". The Journal of Biological Chemistry 271 (9): 4864–71. doi:10.1074/jbc.271.9.4864. PMID 8617757.
- Dougherty, WG; Parks, TD; Cary, SM; Bazan, JF; Fletterick, RJ (September 1989). "Characterization of the catalytic residues of the tobacco etch virus 49-kDa proteinase.". Virology 172 (1): 302–10. doi:10.1016/0042-6822(89)90132-3. PMID 2475971.