Poly(rC)-binding protein 1 is a protein that in humans is encoded by the PCBP1gene.
This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
^Tommerup N, Leffers H (May 1997). "Assignment of human KH-box-containing genes by in situ hybridization: HNRNPK maps to 9q21.32-q21.33, PCBP1 to 2p12-p13, and PCBP2 to 12q13.12-q13.13, distal to FRA12A". Genomics32 (2): 297–8. doi:10.1006/geno.1996.0121. PMID8833161.
Leffers H, Dejgaard K, Celis JE (1995). "Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains.". Eur. J. Biochem.230 (2): 447–53. doi:10.1111/j.1432-1033.1995.tb20581.x. PMID7607214.
Makeyev AV, Chkheidze AN, Liebhaber SA (1999). "A set of highly conserved RNA-binding proteins, alphaCP-1 and alphaCP-2, implicated in mRNA stabilization, are coexpressed from an intronless gene and its intron-containing paralog.". J. Biol. Chem.274 (35): 24849–57. doi:10.1074/jbc.274.35.24849. PMID10455157.
Makeyev AV, Liebhaber SA (2000). "Identification of two novel mammalian genes establishes a subfamily of KH-domain RNA-binding proteins.". Genomics67 (3): 301–16. doi:10.1006/geno.2000.6244. PMID10936052.
Yeap BB, Voon DC, Vivian JP, et al. (2002). "Novel binding of HuR and poly(C)-binding protein to a conserved UC-rich motif within the 3'-untranslated region of the androgen receptor messenger RNA.". J. Biol. Chem.277 (30): 27183–92. doi:10.1074/jbc.M202883200. PMID12011088.
Giles KM, Daly JM, Beveridge DJ, et al. (2003). "The 3'-untranslated region of p21WAF1 mRNA is a composite cis-acting sequence bound by RNA-binding proteins from breast cancer cells, including HuR and poly(C)-binding protein.". J. Biol. Chem.278 (5): 2937–46. doi:10.1074/jbc.M208439200. PMID12431987.
Adams DJ, Beveridge DJ, van der Weyden L, et al. (2004). "HADHB, HuR, and CP1 bind to the distal 3'-untranslated region of human renin mRNA and differentially modulate renin expression.". J. Biol. Chem.278 (45): 44894–903. doi:10.1074/jbc.M307782200. PMID12933794.
Evans JR, Mitchell SA, Spriggs KA, et al. (2003). "Members of the poly (rC) binding protein family stimulate the activity of the c-myc internal ribosome entry segment in vitro and in vivo.". Oncogene22 (39): 8012–20. doi:10.1038/sj.onc.1206645. PMID12970749.