pGLO

From Wikipedia, the free encyclopedia
Jump to: navigation, search
Left: pGLO under ambient light

Right: pGLO visualized under ultraviolet light

The pGLO plasmid is an engineered plasmid used in biotechnology as a vector for creating genetically modified organisms. The plasmid contains several reporter genes, most notably for the green fluorescent protein (GFP) and the ampicillin resistance gene. GFP was isolated from the jelly fish Aequorea victoria. Because it shares a bidirectional promoter with a gene for metabolizing arabinose, the GFP gene is expressed in the presence of arabinose, which makes the transgenic organism fluoresce under UV light. GFP can be induced in bacteria containing the pGLO plasmid by growing them on +arabinose plates.

pGLO is made up of three genes that are joined together using recombinant DNA technology. They are as follows:
-Bla, which codes for the enzyme beta-lactamase giving the transformed bacteria resistance to the beta-lactam family of antibiotics (such as of the penicillin family)
-araC, a promoter region that regulates the expression of GFP (specifically, the GFP gene will be expressed only in the presence of arabinose)
-GFP, the green fluorescent protein, which gives a green glow if cells produce this type of protein

Like most other circular plasmids, the pGLO plasmid contains an origin (ori), which is a region of the plasmid where replication will originate. The pGLO plasmid was made famous by researchers in France who used it to produce a green fluorescent rabbit named Alba.

Other features on pGLO, like most other plasmids, include: a selectable marker, Ori (origin of replication), and an MCS (multiple cloning site) located at the end of the GFP gene. The plasmid is 5371 base pairs long. In supercoiled form, it runs on an agarose gel in the 4200-4500 range.[1][2][3]

References[edit]

  1. ^ Biorad pGlo-Biotechnology [1]
  2. ^ Eichbaum QG, Iyer R, Raveh DP, Mathieu C, Ezekowitz RAB: Restriction of interferon gamma responsiveness and basal expression of myeloid human Fc gamma R1b gene is mediated by a functional PU.1 site and a transcription initiator consensus. J Exp Med 179:1985[2]
  3. ^ Quentin Eichbaum, David Heney, David Raveh, Michael Chung, Mark Davidson, Judith Epstein, and R. Alan B. Ezekowitz Murine Macrophage Mannose Receptor Promoter Is Regulated by the Transcription Factors PU.1 and SP1 Blood, Nov 1997; 90: 4135 - 4143.[3]