DNA polymerase iota is an enzyme that in humans is encoded by the POLIgene. It is found in higher eukaryotes, and is believed to have arisen from a gene duplication from Pol η. Pol ι, is a Y family polymerase that is involved in translesion synthesis. It can bypass 6-4 pyrimidine adducts and abasic sites and has a high frequency of wrong base incorporation. Like many other Y family polymerases Pol ι, has low processivity, a large DNA binding pocket and doesn't undergo conformational changes when DNA binds. These attributes are what allow Pol ι to carry out its task as a translesion polymerase. Pol ι only uses Hoogsteen base pairing, during DNA synthesis, it will add adenine opposite to thymine in the syn conformation and can add both cytosine and thymine in the anti conformation across guanine, which it flips to the syn conformation.
^Frank EG, Woodgate R (Aug 2007). "Increased catalytic activity and altered fidelity of human DNA polymerase iota in the presence of manganese". J Biol Chem282 (34): 24689–96. doi:10.1074/jbc.M702159200. PMID17609217.
Tissier A, Frank EG, McDonald JP et al. (2001). "Biochemical characterization of human DNA polymerase iota provides clues to its biological function.". Biochem. Soc. Trans.29 (Pt 2): 183–7. doi:10.1042/BST0290183. PMID11356150.CS1 maint: Explicit use of et al. (link)
McDonald JP, Rapić-Otrin V, Epstein JA et al. (1999). "Novel human and mouse homologs of Saccharomyces cerevisiae DNA polymerase eta.". Genomics60 (1): 20–30. doi:10.1006/geno.1999.5906. PMID10458907.CS1 maint: Explicit use of et al. (link)
Bebenek K, Tissier A, Frank EG et al. (2001). "5'-Deoxyribose phosphate lyase activity of human DNA polymerase iota in vitro.". Science291 (5511): 2156–9. doi:10.1126/science.1058386. PMID11251121.CS1 maint: Explicit use of et al. (link)
Vaisman A, Tissier A, Frank EG et al. (2001). "Human DNA polymerase iota promiscuous mismatch extension.". J. Biol. Chem.276 (33): 30615–22. doi:10.1074/jbc.M102694200. PMID11402031.CS1 maint: Explicit use of et al. (link)
Faili A, Aoufouchi S, Flatter E et al. (2002). "Induction of somatic hypermutation in immunoglobulin genes is dependent on DNA polymerase iota.". Nature419 (6910): 944–7. doi:10.1038/nature01117. PMID12410315.CS1 maint: Explicit use of et al. (link)