Amidophosphoribosyltransferase

amidophosphoribosyltransferase
Identifiers
EC number	2.4.2.14
CAS number	9031-82-7
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search
PMC	articles
PubMed	articles

Phosphoribosyl pyrophosphate amidotransferase
Identifiers
Symbols	PPAT; ATASE; GPAT; PRAT
External IDs	OMIM: 172450 MGI: 2387203 HomoloGene: 68272 GeneCards: PPAT Gene
EC number	2.4.2.14
Gene Ontology
Molecular function	• amidophosphoribosyltransferase activity; • transferase activity, transferring glycosyl groups; • metal ion binding; • 4 iron, 4 sulfur cluster binding;
Cellular component	• soluble fraction; • cytosol;
Biological process	• purine base metabolic process; • purine nucleotide biosynthetic process; • glutamine metabolic process; • lactation; • purine base biosynthetic process; • nucleoside metabolic process; • purine ribonucleoside monophosphate biosynthetic process; • organ regeneration; • cellular response to insulin stimulus; • protein homotetramerization; • nucleobase, nucleoside and nucleotide metabolic process; • maternal process involved in female pregnancy;
	Sources: Amigo / QuickGO
Orthologs

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Amidophosphoribosyltransferase (ATase), also known as glutamine phosphoribosylpyrophosphate amidotransferase (GPAT), is an enzyme that in humans is encoded by the PPAT (phosphoribosyl pyrophosphate amidotransferase) gene.^[1]^[2]

[edit] Function

ATase is an enzyme that converts α-phosphoribosylpyrophosphate (α-PRPP) into 5-β-phosphoribosylamine. The enzyme uses the ammonia group from the glutamine side-chain. This is the committing step in de novo purine synthesis. It is allosterically inhibited by AMP, GMP, and IMP.

ATase is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis.^[1]

[edit] Interactive pathway map

Click on genes, proteins and metabolites below to link to respective articles. ^[3]

‹ The template below (Css Image Crop) is being considered for deletion. See templates for discussion to help reach a consensus.›

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Fluorouracil (5-FU) Activity edit

[edit] References

^ ^a ^b "Entrez Gene: phosphoribosyl pyrophosphate amidotransferase". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5471.
^ Brayton KA, Chen Z, Zhou G, Nagy PL, Gavalas A, Trent JM, Deaven LL, Dixon JE, Zalkin H (February 1994). "Two genes for de novo purine nucleotide synthesis on human chromosome 4 are closely linked and divergently transcribed". J. Biol. Chem. 269 (7): 5313–21. PMID 8106516.
^ The interactive pathway map can be edited at WikiPathways: "Fluoropyrimidine_Activity_WP1601". http://www.wikipathways.org/index.php/Pathway:WP1601.

[edit] Further reading

Iwahana H, Oka J, Mizusawa N, et al. (1993). "Molecular cloning of human amidophosphoribosyltransferase.". Biochem. Biophys. Res. Commun. 190 (1): 192–200. doi:10.1006/bbrc.1993.1030. PMID 8380692.
Gassmann MG, Stanzel A, Werner S (1999). "Growth factor-regulated expression of enzymes involved in nucleotide biosynthesis: a novel mechanism of growth factor action.". Oncogene 18 (48): 6667–76. doi:10.1038/sj.onc.1203120. PMID 10597272.
Chen S, Nagy PL, Zalkin H (1997). "Role of NRF-1 in bidirectional transcription of the human GPAT-AIRC purine biosynthesis locus.". Nucleic Acids Res. 25 (9): 1809–16. doi:10.1093/nar/25.9.1809. PMC 146651. PMID 9108165. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=146651.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928.
Stanley W, Chu EH (1978). "Assignment of the gene for phosphoribosylpyrophosphate amidotransferase to the pter leads to q21 region of human chromosome 4.". Cytogenet. Cell Genet. 22 (1-6): 228–31. doi:10.1159/000130943. PMID 752480.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
Strausberg RL, Feingold EA, Grouse LH, et al. (2002). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241.
Bera AK, Chen S, Smith JL, Zalkin H (1999). "Interdomain signaling in glutamine phosphoribosylpyrophosphate amidotransferase.". J. Biol. Chem. 274 (51): 36498–504. doi:10.1074/jbc.274.51.36498. PMID 10593947.
Zalkin H, Dixon JE (1992). "De novo purine nucleotide biosynthesis.". Prog. Nucleic Acid Res. Mol. Biol. 42: 259–87. PMID 1574589.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.

[edit] External links

MeSH transferase Amidophosphoribosyl transferase

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

This transferase article is a stub. You can help Wikipedia by expanding it.

[entrez-0] "Entrez Gene: phosphoribosyl pyrophosphate amidotransferase". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=5471.

[pmid8106516-1] Brayton KA, Chen Z, Zhou G, Nagy PL, Gavalas A, Trent JM, Deaven LL, Dixon JE, Zalkin H (February 1994). "Two genes for de novo purine nucleotide synthesis on human chromosome 4 are closely linked and divergently transcribed". J. Biol. Chem. 269 (7): 5313–21. PMID 8106516.

[WikiPathways-2] The interactive pathway map can be edited at WikiPathways: "Fluoropyrimidine_Activity_WP1601". http://www.wikipathways.org/index.php/Pathway:WP1601.

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Amidophosphoribosyltransferase

Contents

[edit] Function

[edit] Interactive pathway map

[edit] References

[edit] Further reading

[edit] External links

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