PPP5C

From Wikipedia, the free encyclopedia
Jump to: navigation, search
Protein phosphatase 5, catalytic subunit
Protein PPP5C PDB 1a17.png
PDB rendering based on 1a17.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols PPP5C ; PP5; PPP5; PPT
External IDs OMIM600658 MGI102666 HomoloGene4550 GeneCards: PPP5C Gene
EC number 3.1.3.16
RNA expression pattern
PBB GE PPP5C 215705 at tn.png
PBB GE PPP5C 201979 s at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 5536 19060
Ensembl ENSG00000011485 ENSMUSG00000003099
UniProt P53041 Q60676
RefSeq (mRNA) NM_001204284 NM_011155
RefSeq (protein) NP_001191213 NP_035285
Location (UCSC) Chr 19:
46.85 – 46.9 Mb
Chr 7:
17 – 17.03 Mb
PubMed search [1] [2]

Serine/threonine-protein phosphatase 5 is an enzyme that in humans is encoded by the PPP5C gene.[1][2]


Model organisms[edit]

Model organisms have been used in the study of PPP5C function. A conditional knockout mouse line, called Ppp5ctm1a(EUCOMM)Wtsi[12][13] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[14][15][16]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[10][17] Twenty five tests were carried out on mutant mice and five significant abnormalities were observed.[10] Homozygous mutant males had decreased body weight, body length and respiratory quotient. Both sexes had increased T cell numbers and a range of skeletal abnormalities identified by radiography.[10]

Interactions[edit]

PPP5C has been shown to interact with ASK1,[18] CRY2[19] GNA12.[20] and Rac1,[21]

References[edit]

  1. ^ Yong WH, Ueki K, Chou D, Reeves SA, von Deimling A, Gusella JF, Mohrenweiser HW, Buckler AJ, Louis DN (August 1996). "Cloning of a highly conserved human protein serine-threonine phosphatase gene from the glioma candidate region on chromosome 19q13.3". Genomics 29 (2): 533–6. doi:10.1006/geno.1995.9972. PMID 8666404. 
  2. ^ "Entrez Gene: PPP5C Protein phosphatase 5, catalytic subunit". 
  3. ^ "Body weight data for Ppp5c". Wellcome Trust Sanger Institute. 
  4. ^ "Dysmorphology data for Ppp5c". Wellcome Trust Sanger Institute. 
  5. ^ "Indirect calorimetry data for Ppp5c". Wellcome Trust Sanger Institute. 
  6. ^ "DEXA data for Ppp5c". Wellcome Trust Sanger Institute. 
  7. ^ "Radiography data for Ppp5c". Wellcome Trust Sanger Institute. 
  8. ^ "Peripheral blood lymphocytes data for Ppp5c". Wellcome Trust Sanger Institute. 
  9. ^ "Citrobacter infection data for Ppp5c". Wellcome Trust Sanger Institute. 
  10. ^ a b c d Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. 
  11. ^ Mouse Resources Portal, Wellcome Trust Sanger Institute.
  12. ^ "International Knockout Mouse Consortium". 
  13. ^ "Mouse Genome Informatics". 
  14. ^ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.  edit
  15. ^ Dolgin E (2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718. 
  16. ^ Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. 
  17. ^ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353. 
  18. ^ Morita, K; Saitoh M, Tobiume K, Matsuura H, Enomoto S, Nishitoh H, Ichijo H (November 2001). "Negative feedback regulation of ASK1 by protein phosphatase 5 (PP5) in response to oxidative stress". EMBO J. (England) 20 (21): 6028–36. doi:10.1093/emboj/20.21.6028. ISSN 0261-4189. PMC 125685. PMID 11689443. 
  19. ^ Zhao, S; Sancar A (November 1997). "Human blue-light photoreceptor hCRY2 specifically interacts with protein serine/threonine phosphatase 5 and modulates its activity". Photochem. Photobiol. (UNITED STATES) 66 (5): 727–31. doi:10.1111/j.1751-1097.1997.tb03214.x. ISSN 0031-8655. PMID 9383998. 
  20. ^ Yamaguchi, Yoshiaki; Katoh Hironori, Mori Kazutoshi, Negishi Manabu (August 2002). "Galpha(12) and Galpha(13) interact with Ser/Thr protein phosphatase type 5 and stimulate its phosphatase activity". Curr. Biol. (England) 12 (15): 1353–8. doi:10.1016/S0960-9822(02)01034-5. ISSN 0960-9822. PMID 12176367. 
  21. ^ Chatterjee, A; Wang L, Armstrong DL, Rossie S (February 2010). "Activated Rac1 GTPase translocates protein phosphatase 5 to the cell membrane and stimulates phosphatase activity in vitro". J. Biol. Chem. 285 (6): 3872–82. doi:10.1074/jbc.M109.088427. PMC 2823530. PMID 19948726. 

Further reading[edit]