PYLIS downstream sequence

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Pyrrolysine insertion sequence 1
Predicted secondary structure of PYLIS 1 RNA.
Symbol PYLIS 1
Rfam RF01982
Other data
RNA type Cis-regulatory element
Domain(s) Archaea

In biology, the PYLIS downstream sequence (PYLIS: pyrrolysine insertion sequence) is a stem-loop structure that appears on some mRNA sequences. This structural motif causes the UAG (amber) stop codon to be translated to the amino acid pyrrolysine instead of ending the protein translation. In archaea, the PYLIS downstream sequence is positioned straight after the UAG codon which is translated as pyrrolysine.[1][2]

See also[edit]


  1. ^ Théobald-Dietrich A, Giegé R, Rudinger-Thirion J (2005). "Evidence for the existence in mRNAs of a hairpin element responsible for ribosome dependent pyrrolysine insertion into proteins". Biochimie 87 (9-10): 813–7. doi:10.1016/j.biochi.2005.03.006. PMID 16164991. 
  2. ^ Zhang Y, Baranov PV, Atkins JF, Gladyshev VN (May 2005). "Pyrrolysine and selenocysteine use dissimilar decoding strategies". J. Biol. Chem. 280 (21): 20740–51. doi:10.1074/jbc.M501458200. PMID 15788401. 

Further reading[edit]

  • Longstaff DG, Blight SK, Zhang L, Green-Church KB, Krzycki JA (January 2007). "In vivo contextual requirements for UAG translation as pyrrolysine". Mol. Microbiol. 63 (1): 229–41. doi:10.1111/j.1365-2958.2006.05500.x. PMID 17140411. 
  • Namy O, Zhou Y, Gundllapalli S et al. (November 2007). "Adding pyrrolysine to the Escherichia coli genetic code". FEBS Lett. 581 (27): 5282–8. doi:10.1016/j.febslet.2007.10.022. PMID 17967457. 
  • Theil Have, C; Zambach, S; Christiansen, H (April 4, 2013). "Effects of using coding potential, sequence conservation and mRNA structure conservation for predicting pyrrolysine containing genes". BMC bioinformatics 14 (1): 118. doi:10.1186/1471-2105-14-118. PMID 23557142. 

External links[edit]