Peanut agglutinin

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Peanut Agglutinin complexed with a di-galactose. PDB entry 2dvd

Peanut agglutinin (PNA) is plant lectin protein derived from the fruits of Arachis hypogaea. Peanut agglutinin may also be referred to as Arachis hypogaea lectin. Lectins recognise and bind particular sugar sequences in carbohydrates; peanut agglutinin binds the carbohydrate sequence Gal-β(1-3)-GalNAc. The name "peanut agglutinin" originates from its ability to stick together (agglutinate) cells, such as neuramidase-treated erythrocytes,[1] which have glycoproteins or glycolipids on their surface which include the Gal-β(1-3)-GalNAc carbohydrate sequence.

Structure[edit]

Legume lectin domain
PDB 1lem EBI.jpg
Structure of the monosaccharide binding site of lentil lectin.[2]
Identifiers
Symbol Lectin_legB
Pfam PF00139
Pfam clan CL0004
InterPro IPR001220
PROSITE PDOC00278
SCOP 1lem
SUPERFAMILY 1lem

The protein is 273 amino acids in length with the first 23 residues acting and a signal peptide which is subsequently cleaved. It has a Uniprot accession of P02872. There are over 20 structures of this protein in the PDB which reveal and all beta-sheet protein with a tetrameric quaternary structure. It is a member of the Lectin_legB PFAM family.

Available Structures of peanut agglutinin

Uses in cell biology and biochemistry[edit]

Because peanut agglutinin specifically binds a particular carbohydrate sequence it finds use in a range of methods for cell biology and biochemistry. For example in PNA-affinity chromatography the binding specificity of peanut agglutinin is used to isolate glycosylated molecules which have the sugar sequence Gal-β(1-3)-GalNAc. Peanut agglutinin activity is inhibited by lactose and galactose which compete for the binding site.

Other uses include:

See also[edit]

References[edit]

  1. ^ "PNA specification sheet Medicago AB:". Retrieved 2010 Mars 14. 
  2. ^ Loris R, Casset F, Bouckaert J, et al. (December 1994). "The monosaccharide binding site of lentil lectin: an X-ray and molecular modelling study". Glycoconj. J. 11 (6): 507–17. doi:10.1007/bf00731301. PMID 7696853.