Peptidoglycan glycosyltransferase

peptidoglycan glycosyltransferase
Identifiers
EC number	2.4.1.129
CAS number	79079-04-2
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles

In enzymology, a peptidoglycan glycosyltransferase (EC 2.4.1.129) is an enzyme that catalyzes the chemical reaction

[GlcNAc-(1->4)-Mur₂Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- diphosphoundecaprenol + GlcNAc-(1->4)-Mur₂Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)- diphosphoundecaprenol $\rightleftharpoons$ [GlcNAc-(1->4)-Mur₂Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n⁺1- diphosphoundecaprenol + undecaprenyl diphosphate

The 4 substrates of this enzyme are [[[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-]], diphosphoundecaprenol, [[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-]], and diphosphoundecaprenol, whereas its 3 products are [[[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-]], diphosphoundecaprenol, and undecaprenyl diphosphate.

This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is undecaprenyldiphospho-(N-acetyl-D-glucosaminyl-(1->4)-(N-acetyl-D-mu ramoylpentapeptide):undecaprenyldiphospho-(N-acetyl-D-glucosaminyl-( 1->4)-N-acetyl-D-muramoylpentapeptide) disaccharidetransferase. Other names in common use include PG-II, bactoprenyldiphospho-N-acetylmuramoyl-(N-acetyl-D-glucosaminyl)-, pentapeptide:peptidoglycan, N-acetylmuramoyl-N-acetyl-D-glucosaminyltransferase, penicillin binding protein (3 or 1B), and peptidoglycan transglycosylase. This enzyme participates in peptidoglycan biosynthesis.

Structural studies

As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes 2BG1, 2UWX, and 2UWY.

References

• Taku A, Stuckey M, Fan DP (1982). "Purification of the peptidoglycan transglycosylase of Bacillus megaterium". J. Biol. Chem. 257 (9): 5018–22. PMID 6802846. 
• Goffin C, Ghuysen JM (1998). "Multimodular penicillin-binding proteins: an enigmatic family of orthologs and paralogs". Microbiol. Mol. Biol. Rev. 62 (4): 1079–93. PMC 98940. PMID 9841666. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=98940. 
• van Heijenoort J (2001). "Formation of the glycan chains in the synthesis of bacterial peptidoglycan". Glycobiology. 11 (3): 25R–36R. doi:10.1093/glycob/11.3.25R. PMID 11320055.