Peridinin

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Peridinin
Skeletal formula
Skeletal formula
Identifiers
CAS number 33281-81-1 YesY
PubChem 5289155
Jmol-3D images Image 1
Properties
Molecular formula C39H50O7
Molar mass 630.81 g mol−1
Except where noted otherwise, data are given for materials in their standard state (at 25 °C (77 °F), 100 kPa)
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Infobox references

Peridinin is a light-harvesting carotenoid, a pigment associated with chlorophyll and found in the peridinin-chlorophyll-protein (PCP) light-harvesting complex in dinoflagellates, best studied in Amphidinium carterae.[1]

Structural characteristics[edit]

The PCP complex is unique in its high ratio of peridinin to chlorophyll; most light-harvesting complexes contain more chlorophyll than carotenoids, but the PCP contains eight peridinin and two chlorophyll molecules arranged to promote peridinin-chlorophyll energy transfer. The PCP complex is a protein trimer with an unusual alpha solenoid protein fold with a molecular weight of 35kDa.

The peridinin-chlorophyll-protein light-harvesting complex. The peridinin molecules are pink and the chlorophyll molecules are black.


Spectral characteristics[edit]

Emission and excitation spectra of Peridinin Chlorophyll (PerCP)
  • Absorption maximum: 483 nm<
  • Emission maximum: 676 nm
  • Extinction coefficient (ε): 1.96 x 106 M−1cm−1
  • A483/A280 ≥ 4.6

Applications[edit]

Peridinin chlorophyll (PerCP) is commonly used in immunoassays such as fluorescence-activated cell sorting (FACS) and flow cytometry. The fluorophore is covalently linked to proteins or antibodies for use in research applications.[2]

References[edit]

  1. ^ Hofmann E, Wrench PM, Sharples FP, Hiller RG, Welte W, Diederichs K (1996). "Structural basis of light harvesting by carotenoids: peridinin-chlorophyll-protein from Amphidinium carterae". Science 272 (5269): 1788–1791. doi:10.1126/science.272.5269.1788. PMID 8650577. 
  2. ^ [1]