Pgrac
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PGRAC = initialed abbreviation for Provincial Grand Royal Arch Chapter. A Masonic Order.
Pgrac is an artificial promoter consisting of Bacillus subtilis groE promoter, lac operator and gsiB ribosome binding site. This is a strong IPTG-inducible promoter for B. subtilis, which was first appeared on plasmid pNDH33[1] and later on pHT01 of the company MoBiTec [2]. Plasmid pHT01 is supposed to be stable in both B. subtilis and Escherichia coli, and can be used for expression of proteins in these host strains. Using pNDH33/pHT01 proteins could be produced up to 16% of total protein in B. subtilis.
[edit] References
- Phan T.P.T., Nguyen H.D. and Schumann W. (2006) Protein Expression and Purification 46: 189-195.
- Nguyen H.D., Phan T.P.T. and Schumann W. (2007) Current Microbiology 55: 89-93.
- Bacillus subtilis Protein Expression System Handbook (2008)[3], [4].
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