PhiKMV-like viruses

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PhiKMV-like viruses
Virus classification
Group: Group I (dsDNA)
Order: Caudovirales
Family: Podoviridae
Subfamily: Autographivirinae
Genus: PhiKMV-like viruses

Phage LKA1
Phage LKD16
Phage LUZ19
Phage phiKMV

PhiKMV-like viruses are a genus of Autographivirinae viruses. Bacteriophage phiKMV[1] and its relatives are known to be highly virulent phages, producing large (3–15 mm (0.12–0.59 in) diameter) clear plaques upon plating on a susceptible host.[2][3] The only reported exception is phage LKA1, which yields small plaques (1 mm (0.039 in)) surrounded by a halo. While all other P. aeruginosa-specific phiKMV-like viruses use the Type IV pili as primary receptor, LKA1 particles attach to the bacterial lipopolysacharide layer.


Electron microscopic imaging of purified phage particles revealed these phages as typical members of the Podoviridae, with a head diameter of approximately 60 nm (2.4×10−6 in) and a stubby tail with a length of 8–10 nm. Although phiKMV phage resembles the well-studied podovirus T7 in overall genome architecture, it was the first known T7-like phage which encoded a single-subunit RNA polymerase gene downstream its DNA metabolism genes instead of in the early genomic region. Based on these properties, the "phiKMV-like viruses" genus is classified within the Autographivirinae.[4]


  1. ^ Lavigne, Rob; Burkal'tseva, Maria V.; Robben, Johan; Sykilinda, Nina N.; Kurochkina, Lidia P.; Grymonprez, Barbara; Jonckx, Bart; Krylov, Victor N. et al. (2003). "The genome of bacteriophage φKMV, a T7-like virus infecting Pseudomonas aeruginosa". Virology 312 (1): 49–59. doi:10.1016/S0042-6822(03)00123-5. PMID 12890620. 
  2. ^ Ceyssens, P.-J.; Lavigne, R.; Mattheus, W.; Chibeu, A.; Hertveldt, K.; Mast, J.; Robben, J.; Volckaert, G. (2006). "Genomic Analysis of Pseudomonas aeruginosa Phages LKD16 and LKA1: Establishment of the  KMV Subgroup within the T7 Supergroup". Journal of Bacteriology 188 (19): 6924–31. doi:10.1128/JB.00831-06. PMC 1595506. PMID 16980495. 
  3. ^ Lammens, E.; Ceyssens, P.-J.; Voet, M.; Hertveldt, K.; Lavigne, R.; Volckaert, G. (2009). "Representational Difference Analysis (RDA) of bacteriophage genomes". Journal of Microbiological Methods 77 (2): 207–13. doi:10.1016/j.mimet.2009.02.006. PMID 19232531. 
  4. ^ Lavigne, Rob; Seto, Donald; Mahadevan, Padmanabhan; Ackermann, Hans-W.; Kropinski, Andrew M. (2008). "Unifying classical and molecular taxonomic classification: analysis of the Podoviridae using BLASTP-based tools". Research in Microbiology 159 (5): 406–14. doi:10.1016/j.resmic.2008.03.005. PMID 18555669.