PhiKMV-like viruses

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PhiKMV-like viruses
Virus classification
Group: Group I (dsDNA)
Order: Caudovirales
Family: Podoviridae
Subfamily: Autographivirinae
Genus: PhiKMV-like viruses
Species

Phage LKA1
Phage LKD16
Phage LUZ19
Phage phiKMV

PhiKMV-like viruses are a genus of Autographivirinae viruses. Bacteriophage phiKMV[1] and its relatives are known to be highly virulent phages, producing large (3–15 mm (0.12–0.59 in) diameter) clear plaques upon plating on a susceptible host.[2][3] The only reported exception is phage LKA1, which yields small plaques (1 mm (0.039 in)) surrounded by a halo. While all other P. aeruginosa-specific phiKMV-like viruses use the Type IV pili as primary receptor, LKA1 particles attach to the bacterial lipopolysacharide layer.

Virology[edit]

Electron microscopic imaging of purified phage particles revealed these phages as typical members of the Podoviridae, with a head diameter of approximately 60 nm (2.4×10−6 in) and a stubby tail with a length of 8–10 nm. Although phiKMV phage resembles the well-studied podovirus T7 in overall genome architecture, it was the first known T7-like phage which encoded a single-subunit RNA polymerase gene downstream its DNA metabolism genes instead of in the early genomic region. Based on these properties, the "phiKMV-like viruses" genus is classified within the Autographivirinae.[4]

References[edit]

  1. ^ Lavigne, Rob; Burkal'tseva, Maria V.; Robben, Johan; Sykilinda, Nina N.; Kurochkina, Lidia P.; Grymonprez, Barbara; Jonckx, Bart; Krylov, Victor N. et al. (2003). "The genome of bacteriophage φKMV, a T7-like virus infecting Pseudomonas aeruginosa". Virology 312 (1): 49–59. doi:10.1016/S0042-6822(03)00123-5. PMID 12890620. 
  2. ^ Ceyssens, P.-J.; Lavigne, R.; Mattheus, W.; Chibeu, A.; Hertveldt, K.; Mast, J.; Robben, J.; Volckaert, G. (2006). "Genomic Analysis of Pseudomonas aeruginosa Phages LKD16 and LKA1: Establishment of the  KMV Subgroup within the T7 Supergroup". Journal of Bacteriology 188 (19): 6924–31. doi:10.1128/JB.00831-06. PMC 1595506. PMID 16980495. 
  3. ^ Lammens, E.; Ceyssens, P.-J.; Voet, M.; Hertveldt, K.; Lavigne, R.; Volckaert, G. (2009). "Representational Difference Analysis (RDA) of bacteriophage genomes". Journal of Microbiological Methods 77 (2): 207–13. doi:10.1016/j.mimet.2009.02.006. PMID 19232531. 
  4. ^ Lavigne, Rob; Seto, Donald; Mahadevan, Padmanabhan; Ackermann, Hans-W.; Kropinski, Andrew M. (2008). "Unifying classical and molecular taxonomic classification: analysis of the Podoviridae using BLASTP-based tools". Research in Microbiology 159 (5): 406–14. doi:10.1016/j.resmic.2008.03.005. PMID 18555669.