Platelet lysate
From Wikipedia, the free encyclopedia
Platelet lysate (or PL) is as a substitute supplement for fetal bovine serum (FBS) in experimental cell culture. It corresponds to a light-yellow liquid that is obtained from blood platelets after freeze/thaw process, which contains a huge quantity of growth factors necessary for cell expansion.
[edit] Process
PL is prepared from platelets isolated from whole blood distributed in a standard platelet collection bag. There are some differences between protocols, but they share the same core of being frozen at very low temperatures and thawed on the day after. This process is repeated three or four times and the remained supernatant is purified and stored at -20°C
[edit] Source history
In 1991, the U.S. Food and Drug Administration (FDA) recalled two lots of FBS processed in the US. The serum had arrived from Brazil labelled as human serum and thereby evading the U.S. Department of Agriculture. The source of FBS and all serum is critical when considering the purchase of serum. The source now determines whether or not a biotherapeutic agent will receive approval by certain regulatory bodies including the USDA.
[edit] Global sales
PL is not available for sale.
[edit] See also
Laboratory use of serum
[edit] References
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