PrfA thermoregulator UTR

From Wikipedia, the free encyclopedia
Jump to: navigation, search
PrfA thermoregulator UTR
Predicted secondary structure and sequence conservation of PrfA
Symbol PrfA
Rfam RF00038
Other data
RNA type Cis-reg; thermoregulator
Domain(s) Bacteria
SO 0000233

The PrfA thermoregulator UTR is an RNA thermometer found in the 5' UTR of the prfA gene.[1] In Listeria monocytogenes, virulence genes are maximally expressed at 37°C (human body temperature) but are almost silent at 30°C.[2] The genes are controlled by PrfA, a transcriptional activator whose expression is thermoregulated.[3] It has been shown that the untranslated mRNA (UTR) preceding prfA, forms a secondary structure, which masks the ribosome binding region.[1] It is thought that at 37°C, the hairpin structure 'melts' and the SD sequence is unmasked.[1]


  1. ^ a b c Johansson J, Mandin P, Renzoni A, Chiaruttini C, Springer M, Cossart P (2002). "An RNA thermosensor controls expression of virulence genes in Listeria monocytogenes". Cell 110 (5): 551–61. doi:10.1016/S0092-8674(02)00905-4. PMID 12230973. 
  2. ^ Freitag, NE; Port, GC, Miner, MD (September 2009). "Listeria monocytogenes - from saprophyte to intracellular pathogen.". Nature reviews. Microbiology 7 (9): 623–8. doi:10.1038/nrmicro2171. PMC 2813567. PMID 19648949. Retrieved 10 August 2011. 
  3. ^ Digel, I; Kayser, P; Artmann, GM (2008). "Molecular processes in biological thermosensation.". Journal of biophysics (Hindawi Publishing Corporation : Online) 2008: 602870. doi:10.1155/2008/602870. PMC 2814129. PMID 20130806. Retrieved 10 August 2011. 

External links[edit]