RF3 promotes termination by either factor by accelerating dissociation.
The release factors provide the means of termination by having a conformation that mimics that of tRNA molecules. The decoding release factor binds to the A site of the ribosome and directly recognises the stop codon. Once RF1 (or RF2) and RF3 are bound to the ribosome, the polypeptide is released, and the ribosome and release factors disassemble, thus completing the process of translation.
^Brown, CM; Tate, WP (1994). "Direct recognition of mRNA stop signals by Escherichia coli polypeptide chain release factor two". The Journal of Biological Chemistry269 (52): 33164–70. PMID7806547.
^Scarlett, D.-J. G.; McCaughan, KK; Wilson, DN; Tate, WP (2002). "Mapping Functionally Important Motifs SPF and GGQ of the Decoding Release Factor RF2 to the Escherichia coli Ribosome by Hydroxyl Radical Footprinting. IMPLICATIONS FOR MACROMOLECULAR MIMICRY AND STRUCTURAL CHANGES IN RF2". Journal of Biological Chemistry278 (17): 15095–15104. doi:10.1074/jbc.M211024200. PMID12458201.