A protein kinase is a kinase enzyme that modifies other proteins by chemically adding phosphate groups to them (phosphorylation). Phosphorylation usually results in a functional change of the target protein (substrate) by changing enzyme activity, cellular location, or association with other proteins. The human genome contains about 500 protein kinase genes and they constitute about 2% of all human genes. Up to 30% of all human proteins may be modified by kinase activity, and kinases are known to regulate the majority of cellular pathways, especially those involved in signal transduction. Protein kinases are also found in bacteria and plants.
Chemical activity 
The chemical activity of a kinase involves transferring a phosphate group from a nucleoside triphosphate (usually ATP) and covalently attaching it to specific amino acids with a free hydroxyl group. Most kinases act on both serine and threonine (serine/threonine kinases), others act on tyrosine (tyrosine kinases), and a number act on all three (dual-specificity kinases). There are also protein kinases that phosphorylate other amino acids, including histidine kinases that phosphorylate histidine residues.
Because protein kinases have profound effects on a cell, their activity is highly regulated. Kinases are turned on or off by phosphorylation (sometimes by the kinase itself - cis-phosphorylation/autophosphorylation), by binding of activator proteins or inhibitor proteins, or small molecules, or by controlling their location in the cell relative to their substrates.
Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins that share a conserved catalytic core. There are a number of conserved regions in the catalytic domain of protein kinases. In the N-terminal extremity of the catalytic domain there is a glycine-rich stretch of residues in the vicinity of a lysine amino acid, which has been shown to be involved in ATP binding. In the central part of the catalytic domain, there is a conserved aspartic acid, which is important for the catalytic activity of the enzyme.
Protein kinase groups 
The human protein kinase family is divided into the following groups:
- AGC kinases - containing PKA, PKC and PKG.
- CaM kinases - containing the calcium/calmodulin-dependent protein kinases.
- CK1 - containing the casein kinase 1 group.
- CMGC - containing CDK, MAPK, GSK3 and CLK kinases.
- STE - containing the homologs of yeast Sterile 7, Sterile 11, and Sterile 20 kinases.
- TK - containing the tyrosine kinases.
- TKL - containing the tyrosine-kinase like group of kinases.
Serine/threonine-specific protein kinases 
Serine/threonine protein kinases (EC 184.108.40.206) phosphorylate the OH group of serine or threonine (which have similar side-chains). Activity of these protein kinases can be regulated by specific events (e.g., DNA damage), as well as numerous chemical signals, including cAMP/cGMP, diacylglycerol, and Ca2+/calmodulin. One very important group of protein kinases are the MAP kinases (acronym from: "mitogen-activated protein kinases"). Important subgroups are the kinases of the ERK subfamily, typically activated by mitogenic signals, and the stress-activated protein kinases JNK and p38. While MAP kinases are serine/threonine-specific, they are activated by combined phosphorylation on serine/threonine and tyrosine residues. Activity of MAP kinases is restricted by a number of protein phosphatases, which remove the phosphate groups that are added to specific serine or threonine residues of the kinase and are required to maintain the kinase in an active conformation. Two major factors influence activity of MAP kinases: a) signals that activate transmembrane receptors (either natural ligands or crosslinking agents) and proteins associated with them (mutations that simulate active state) b) signals that inactivate the phosphatases that restrict a given MAP kinase. Such signals include oxidant stress.
Tyrosine-specific protein kinases 
Tyrosine-specific protein kinases (EC 220.127.116.11 and EC 18.104.22.168) phosphorylate tyrosine amino acid residues, and like serine/threonine-specific kinases are used in signal transduction. They act primarily as growth factor receptors and in downstream signaling from growth factors; some examples:
- Platelet-derived growth factor receptor (PDGFR)
- Epidermal growth factor receptor (EGFR)
- Insulin receptor and insulin-like growth factor 1 receptor (IGF1R)
- Stem cell factor (SCF) receptor (also called c-kit, see the article on gastrointestinal stromal tumor).
Receptor tyrosine kinases 
These kinases consist of a transmembrane receptor with a tyrosine kinase domain protruding into the cytoplasm. They play an important role in regulating cell division, cellular differentiation, and morphogenesis. More than 50 receptor tyrosine kinases are known in mammals.
The extracellular domain serves as the ligand-binding part of the molecule. It can be a separate unit that is attached to the rest of the receptor by a disulfide bond. The same mechanism can be used to bind two receptors together to form a homo- or heterodimer. The transmembrane element is a single α helix. The intracellular or cytoplasmic domain is responsible for the (highly conserved) kinase activity, as well as several regulatory functions.
Ligand binding causes two reactions:
- Dimerization of two monomeric receptor kinases or stabilization of a loose dimer. Many ligands of receptor tyrosine kinases are multivalent. Some tyrosine receptor kinases (e.g., the platelet-derived growth factor receptor) can form heterodimers with other similar but not identical kinases of the same subfamily, allowing a highly varied response to the extracellular signal.
- Trans-autophosphorylation (phosphorylation by the other kinase in the dimer or higher order multimer complex) of the kinase.
The autophosphorylation causes the two subdomains of the intrinsic kinase to shift, opening the kinase domain for ATP binding. In the inactive form, the kinase subdomains are aligned so that ATP cannot reach the catalytic center of the kinase. When several amino acids suitable for phosphorylation are present in the kinase domain (e.g., the insulin-like growth factor receptor), the activity of the kinase can increase with the number of phosphorylated amino acids; in this case, the first phosphorylation is said to be a cis-autophosphorylation, switching the kinase from "off" to "standby".
Signal transduction 
The active tyrosine kinase phosphorylates specific target proteins, which are often enzymes themselves. An important target is the ras protein signal-transduction chain.
Receptor-associated tyrosine kinases 
Tyrosine kinases recruited to a receptor following hormone binding are receptor-associated tyrosine kinases and are involved in a number of signaling cascades, in particular those involved in cytokine signaling (but also others, including growth hormone). One such receptor-associated tyrosine kinase is Janus kinase (JAK), many of whose effects are mediated by STAT proteins. (See JAK-STAT pathway.)
Histidine-specific protein kinases 
Histidine kinases are structurally distinct from most other protein kinases and are found mostly in prokaryotes as part of two-component signal transduction mechanisms. A phosphate group from ATP is first added to a histidine residue within the kinase, and later transferred to an aspartate residue on a 'receiver domain' on a different protein, or sometimes on the kinase itself. The aspartyl phosphate residue is then active in signaling.
Histidine kinases are also found in plants, fungi, animals, and other eukaryotes. The pyruvate dehydrogenase family of kinases in animals is structurally related to histidine kinases, but instead phosphorylate serine residues, and probably do not use a phospho-histidine intermediate.
Mixed kinases 
Some kinases have mixed kinase activities. For example, MEK (MAPKK), which is involved in the MAP kinase cascade, is a mixed serine/threonine and tyrosine kinase and, hence, is a dual-specificity kinase.
Deregulated kinase activity is a frequent cause of disease, in particular cancer, wherein kinases regulate many aspects that control cell growth, movement and death. Drugs that inhibit specific kinases are being developed to treat several diseases, and some are currently in clinical use, including Gleevec (imatinib) and Iressa (gefitinib).
Kinase assays and profiling 
Drug developments for kinase inhibitors are started from kinase assays, the lead compounds are usually profiled for specificity before moving into further tests. Many profiling services are available from fluorescent-based assays like the microscale thermophoresis assay to radioisotope based detections, and competition binding assays.
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- List of protein phosphorylation databases and other related resources
- The Protein Kinase Resource: Curated database of protein kinase structures and related data
- Human and mouse protein kinases: classification and index
- The Kinase Knowledgebase (KKB): Database of kinase structure-activity and chemical synthesis data.
- Kinase.Com: Genomics, evolution and large-scale analysis of protein kinases (non-commercial).
- Kinase/TIP: Database containing thousands of protein structures, co-complexes and models surveying the Human Kinome.
- AurSCOPE Kinase Database
- Kinasecentral: Information on Kinase inhibitors in development
- Collection of Ser/Thr/Tyr specific protein kinases and similar sequences
- KinMutBase: A registry of disease-causing mutations in protein kinase domains
- Human kinome by Manning et al.
- UMich Orientation of Proteins in Membranes families/superfamily-63 - Orientations of C1 domains of protein kinases in membranes
- UMich Orientation of Proteins in Membranes families/superfamily-47 - Orientations of C2 domains of protein kinases and other proteins in membranes
- Huaxian Chen, et al. A Cell Based Immunochemical Assay for Monitoring Kinase Signaling Pathways and Drug Efficacy (PDF) Analytical Biochemistry 338 (2005) 136-142
- MAP Kinase Resource