|P. mirabilis on an XLD agar plate.|
P. mirabilis can be diagnosed in the lab due to characteristic swarming motility, and inability to metabolize lactose (on a MacConkey agar plate, for example). Also P. mirabilis produces a very distinct fishy odour.
This rod shaped bacterium has the ability to produce high levels of urease. Urease hydrolyzes urea to ammonia (NH3) and thus makes the urine more alkaline. If left untreated, the increased alkalinity can lead to the formation of crystals of struvite, calcium carbonate, and/or apatite. The bacteria can be found throughout the stones, and these bacteria lurking in the kidney stones can reinitiate infection after antibiotic treatment. Once the stones develop, over time they may grow large enough to cause obstruction and renal failure. Proteus can also cause wound infections, septicemia, and pneumonias, mostly in hospitalized patients.
P. mirabilis can use urea. It can produce hydrogen sulfide gas, and forms clear films on growth media. It is motile, possessing peritrichous flagella, and is known for its swarming ability. It is commonly found in the intestinal tract of humans. P. mirabilis is not pathogenic in guinea pigs or chickens. Noteworthy is the ability of this species to inhibit growth of unrelated strains resulting in a macroscopically visible line of reduced bacterial growth where two swarming strains intersect. This line is named Dienes line after its discoverer Louis Dienes.
The micro-organism tests:
- Indole negative and Nitrate reductase positive (no gas bubbles produced)
- Methyl Red positive and Voges-Proskauer negative (Can be both MR and V-P Positive)
- Catalase positive and Cytochrome Oxidase negative
- Phenylalanine Deaminase positive
- Tryptophan test- negative (-)
- Urea test- positive
- Casein test-negative
- Starch test- negative
- Hydrogen sulfide test- positive
- Citrate agar test- Positive
- Ornithine Decarboxylase - Positive
- Lysine Decarboxylase - Negative
- "Proteus mirabilis and Urinary Tract Infection" .
- Proteus Genome Projects from Genomes OnLine Database
- Bacteria of the species Proteus mirabilis are widely distributed in soil and water in the natural environment. In humans, Proteus is found as part of the normal flora of the gut....from http://www.biomedhtc.org.uk/ProteusMirabilis.htm BioMedHTC]
- Esipov, Sergei E.; Shapiro, J. A. (1998). "Kinetic model of Proteus mirabilis swarm colony development". Journal of Mathematical Biology 36 (3): 249. doi:10.1007/s002850050100.
- Frénod, Emmanuel (2006). "Existence result for a model of Proteus mirabilis swarm". Differential and Integral Equations 19 (6): 697–720. arXiv:math.FA/0702761.
- Gué, Michaël; Dupont, Virginie; Dufour, Alain; Sire, Olivier (2001). "Bacterial swarming: A biological time-resolved FTIR-ATR study of Proteus mirabilis swarm-cell differentiation". Biochemistry 40 (39): 11938–11945. doi:10.1021/bi010434m. PMID 11570895.
- Rauprich, O.; Matsushita, M.; Weijer, C. J.; Siegert, F.; Esipov, S. E.; Shapiro, J. A. (1 November 1996). "Periodic phenomena in Proteus mirabilis swarm colony development". Journal of Bacteriology 178 (22): 6525–6538. PMC 178539. PMID 8932309.