Bacillus fluorescens putidus" Flügge 1886
Pseudomonas putida is a Gram-negative, rod-shaped, saprotrophic soil bacterium. Based on 16S rRNA analysis, P. putida was taxonomically confirmed to be a pseudomonas species (sensu stricto) and placed, along with several other species, in the P. putida group, to which it lends its name.
A variety of P. putida, called "multi-plasmid hydrocarbon-degrading Pseudomonas," is the first patented organism in the world. Because it is a living organism, the patent was disputed and brought before the United States Supreme Court in the historic court case Diamond v. Chakrabarty which the inventor, Ananda Mohan Chakrabarty, won. It demonstrates a very diverse metabolism, including the ability to degrade organic solvents such as toluene. This ability has been put to use in bioremediation, or the use of microorganisms to biodegrade oil. Use of P. putida is preferable to some other Pseudomonas species capable of such degradation, as it is a safe species of bacteria, unlike P. aeruginosa, for example, which is an opportunistic human pathogen.
The diverse metabolism of wild-type strains of P. putida may be exploited for bioremediation; for example, it has been shown in the laboratory to function as a soil inoculant to remedy naphthalene-contaminated soils.
However promising the plasmid-engineered variety of the bacterium appears in the laboratory, as Dr. Chakrabarty pointed out in his 2002 interview, "The bacteria, by itself is non-toxic but once in the open-environment it can combine with pathogenic elements and show undesirable results.As there is no-recall once the Pseudomonas is released in the open sea, the utility of the bacteria still remains ambiguous."
Oligonucleotide usage signatures of the P. putida KT2440 genome
Di- to pentanucleotide usage and the list of the most abundant octa- to tetradecanucleotides are useful measures of the bacterial genomic signature. The P. putida KT2440 chromosome is characterized by strand symmetry and intrastrand parity of complementary oligonucleotides. Each tetranucleotide occurs with similar frequency on the two strands. Tetranucleotide usage is biased by G+C content and physicochemical constraints such as base stacking energy, dinucleotide propeller twist angle, or trinucleotide bendability. The 105 regions with atypical oligonucleotide composition can be differentiated by their patterns of oligonucleotide usage into categories of horizontally acquired gene islands, multidomain genes or ancient regions such as genes for ribosomal proteins and RNAs. A species-specific extragenic palindromic sequence is the most common repeat in the genome that can be exploited for the typing of P. putida strains. In the coding sequence of P. putida, LLL is the most abundant tripeptide.
P. Putida's amenability to genetic manipulation has allowed it to be used in the synthesis of numerous organic pharmaceutical and agricultural compounds from various substrates. 
CBB5 and caffeine consumption
- Anzai, et al.; Kim, H; Park, JY; Wakabayashi, H; Oyaizu, H (Jul 2000). "Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence". Int J Syst Evol Microbiol 50 (4): 1563–89. doi:10.1099/00207713-50-4-1563. PMID 10939664.
- Marqués, Silvia; Ramos, Juan L. (1993). "Transcriptional control of the Pseudomonas putida TOL plasmid catabolic pathways". Molecular Microbiology 9 (5): 923–9. doi:10.1111/j.1365-2958.1993.tb01222.x. PMID 7934920.
- Gomes, NC; Kosheleva, IA; Abraham, WR; Smalla, K (2005). "Effects of the inoculant strain Pseudomonas putida KT2442 (pNF142) and of naphthalene contamination on the soil bacterial community". FEMS microbiology ecology 54 (1): 21–33. doi:10.1016/j.femsec.2005.02.005. PMID 16329969.
- Immortal Polystyrene Foam Meets its Enemy | LiveScience
- Ward, PG; Goff, M; Donner, M; Kaminsky, W; O'Connor, KE (2006). "A two step chemo-biotechnological conversion of polystyrene to a biodegradable thermoplastic". Environmental science & technology 40 (7): 2433–7. doi:10.1021/es0517668. PMID 16649270.
- Amer, GA; Utkhede, RS (2000). "Development of formulations of biological agents for management of root rot of lettuce and cucumber". Canadian journal of microbiology 46 (9): 809–16. doi:10.1139/w00-063. PMID 11006841.
- Validov, S; Kamilova, F; Qi, S; Stephan, D; Wang, JJ; Makarova, N; Lugtenberg, B (2007). "Selection of bacteria able to control Fusarium oxysporum f. Sp. Radicis-lycopersici in stonewool substrate". Journal of applied microbiology 102 (2): 461–71. doi:10.1111/j.1365-2672.2006.03083.x. PMID 17241352.
- Cornelis P (editor). (2008). Pseudomonas: Genomics and Molecular Biology (1st ed.). Caister Academic Press. ISBN 1-904455-19-0.
- Summers, RM; Louie, TM; Yu, CL; Subramanian, M (2011). "Characterization of a broad-specificity non-haem iron N-demethylase from Pseudomonas putida CBB5 capable of utilizing several purine alkaloids as sole carbon and nitrogen source". Microbiology (Reading, England) 157 (Pt 2): 583–92. doi:10.1099/mic.0.043612-0. PMID 20966097.
- Risk Assessment Summary, CEPA 1999. Pseudomonas putida CR30RNSLL(pADPTel).
- Pseudomonas putida is an example for plant growth promoting Rhizobacterium,which produces iron chelating substances.