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Puff may refer to:
- Puff, a light gust of wind
- Puff, inhalation
- Puff piece or Puffery, exaggerated advertising claims
- Puff sleeve
- Puff Daddy, or Sean Combs, rapper
- Puff Johnson, American singer
- Puffy AmiYumi, Japanese singer
- Puff Kuo, Taiwanese singer and actress
- Puff, a character in the song "Puff, the Magic Dragon"
- "Puff, the Magic Dragon", nickname for the Douglas AC-47 Spooky U.S. Airforce ground-attack aircraft
Foods with high air content
- Savoury puffs, extruded corn snacks, generic cheese puffs and other flavors
- Curry puff
- Cream puff, profiterole
- Puff pastry
- Puffed grain
- Cocoa Puffs, a brand of chocolate-flavored puffed grain breakfast cereal, manufactured by General Mills
- Sugar Puffs, a brand of sugar-frosted puffed grain breakfast cereal
Low-density materials and products
- Powder puff, face-powder applicator
- Puffs (facial tissue), an American brand of facial tissue from Procter & Gamble
- "Puffs", common term for Chromosome puff, diffused uncoiled regions of the polytene chromosome that are sites of RNA transcription in genetics.
- PUFFS (NetBSD), a NetBSD kernel subsystem developed for running filesystems in userspace
- Puff model, volcanic ash tracking model developed at the University of Alaska Fairbanks
- picofarad (pF), a capacitance unit, sometimes pronounced as "puff"
- PF Debate, or public forum debate, sometimes pronounced as "puff"
- "Puff", also "Puff, the Magic Dragon" song performed by Peter, Paul and Mary, 1963
- Pouf and pouffe (homonyms)
- Puff adder (disambiguation)
- Puffer (disambiguation)
- Puffy (disambiguation)
- The Powerpuff Girls
- Puff Guo
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