RACGAP1

Rac GTPase activating protein 1
PDB rendering based on 2ovj.
Available structures PDB 2OVJ
Identifiers
Symbols	RACGAP1; CYK4; HsCYK-4; ID-GAP; MgcRacGAP
External IDs	OMIM: 604980 MGI: 1349423 HomoloGene: 8077 GeneCards: RACGAP1 Gene
Gene Ontology Molecular function • GTPase activator activity • GTPase activator activity • protein binding • microtubule binding • protein kinase binding • alpha-tubulin binding • gamma-tubulin binding • metal ion binding • beta-tubulin binding Cellular component • acrosomal vesicle • nucleus • cytoplasm • cytosol • cytoskeleton • microtubule • midbody • cytoplasmic vesicle • cleavage furrow • spindle midzone • mitotic spindle • mitotic spindle • centralspindlin complex Biological process • cytokinesis • cytokinesis, actomyosin contractile ring assembly • ion transport • microtubule-based movement • cell cycle • cytokinesis, initiation of separation • small GTPase mediated signal transduction • spermatogenesis • neuroblast proliferation • blood coagulation • sulfate transport • embryo development • cell differentiation • cell cycle cytokinesis • positive regulation of GTPase activity • positive regulation of GTPase activity • regulation of small GTPase mediated signal transduction • spindle midzone assembly involved in mitosis • regulation of attachment of spindle microtubules to kinetochore • positive regulation of cell cycle cytokinesis • positive regulation of cell cycle cytokinesis Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	29127	26934
Ensembl	ENSG00000161800	ENSMUSG00000023015
UniProt	Q9H0H5	Q9WVM1
RefSeq (mRNA)	NM_001126103.1	NM_001253808.1
RefSeq (protein)	NP_001119575.1	NP_001240737.1
Location (UCSC)	Chr 12: 50.37 – 50.43 Mb	Chr 15: 99.45 – 99.48 Mb
PubMed search	[1]	[2]

Rac GTPase-activating protein 1 is an enzyme that in humans is encoded by the RACGAP1 gene.

Rho GTPases control a variety of cellular processes. There are 3 subtypes of Rho GTPases in the Ras superfamily of small G proteins: RHO (see MIM 165370), RAC (see RAC1; MIM 602048), and CDC42 (MIM 116952). GTPase-activating proteins (GAPs) bind activated forms of Rho GTPases and stimulate GTP hydrolysis. Through this catalytic function, Rho GAPs negatively regulate Rho-mediated signals. GAPs may also serve as effector molecules and play a role in signaling downstream of Rho and other Ras-like GTPases.[supplied by OMIM]

Interactions

RACGAP1 has been shown to interact with Rnd2 and SLC26A8.

Further reading

• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298. 
• Gerwins P, Blank JL, Johnson GL (1997). "Cloning of a novel mitogen-activated protein kinase kinase kinase, MEKK4, that selectively regulates the c-Jun amino terminal kinase pathway.". J. Biol. Chem. 272 (13): 8288–95. doi:10.1074/jbc.272.13.8288. PMID 9079650. 
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. 
• Nagata K, Puls A, Futter C, et al. (1998). "The MAP kinase kinase kinase MLK2 co-localizes with activated JNK along microtubules and associates with kinesin superfamily motor KIF3.". EMBO J. 17 (1): 149–58. doi:10.1093/emboj/17.1.149. PMC 1170366. PMID 9427749. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1170366. 
• Tapon N, Nagata K, Lamarche N, Hall A (1998). "A new rac target POSH is an SH3-containing scaffold protein involved in the JNK and NF-kappaB signalling pathways.". EMBO J. 17 (5): 1395–404. doi:10.1093/emboj/17.5.1395. PMC 1170487. PMID 9482736. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1170487. 
• Abo A, Qu J, Cammarano MS, et al. (1999). "PAK4, a novel effector for Cdc42Hs, is implicated in the reorganization of the actin cytoskeleton and in the formation of filopodia.". EMBO J. 17 (22): 6527–40. doi:10.1093/emboj/17.22.6527. PMC 1171000. PMID 9822598. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1171000. 
• Zuber J, Tchernitsa OI, Hinzmann B, et al. (2000). "A genome-wide survey of RAS transformation targets.". Nat. Genet. 24 (2): 144–52. doi:10.1038/72799. PMID 10655059. 
• Das B, Shu X, Day GJ, et al. (2000). "Control of intramolecular interactions between the pleckstrin homology and Dbl homology domains of Vav and Sos1 regulates Rac binding.". J. Biol. Chem. 275 (20): 15074–81. doi:10.1074/jbc.M907269199. PMID 10748082. 
• Nagase T, Kikuno R, Ishikawa K, et al. (2000). "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.". DNA Res. 7 (2): 143–50. doi:10.1093/dnares/7.2.143. PMID 10819331. 
• Kawashima T, Hirose K, Satoh T, et al. (2000). "MgcRacGAP is involved in the control of growth and differentiation of hematopoietic cells.". Blood 96 (6): 2116–24. PMID 10979956. 
• Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination.". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=310948. 
• Hirose K, Kawashima T, Iwamoto I, et al. (2001). "MgcRacGAP is involved in cytokinesis through associating with mitotic spindle and midbody.". J. Biol. Chem. 276 (8): 5821–8. doi:10.1074/jbc.M007252200. PMID 11085985. 
• Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=311072. 
• Caloca MJ, Wang H, Delemos A, et al. (2001). "Phorbol esters and related analogs regulate the subcellular localization of beta 2-chimaerin, a non-protein kinase C phorbol ester receptor.". J. Biol. Chem. 276 (21): 18303–12. doi:10.1074/jbc.M011368200. PMID 11278894. 
• Toure A, Morin L, Pineau C, et al. (2001). "Tat1, a novel sulfate transporter specifically expressed in human male germ cells and potentially linked to rhogtpase signaling.". J. Biol. Chem. 276 (23): 20309–15. doi:10.1074/jbc.M011740200. PMID 11278976. 
• Mishima M, Kaitna S, Glotzer M (2002). "Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity.". Dev. Cell 2 (1): 41–54. doi:10.1016/S1534-5807(01)00110-1. PMID 11782313. 
• Vikis HG, Li W, Guan KL (2002). "The plexin-B1/Rac interaction inhibits PAK activation and enhances Sema4D ligand binding.". Genes Dev. 16 (7): 836–45. doi:10.1101/gad.966402. PMC 186329. PMID 11937491. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=186329. 
• Kitamura T, Kawashima T, Minoshima Y, et al. (2002). "Role of MgcRacGAP/Cyk4 as a regulator of the small GTPase Rho family in cytokinesis and cell differentiation.". Cell Struct. Funct. 26 (6): 645–51. doi:10.1247/csf.26.645. PMID 11942621. 
• Côté JF, Vuori K (2003). "Identification of an evolutionarily conserved superfamily of DOCK180-related proteins with guanine nucleotide exchange activity.". J. Cell. Sci. 115 (Pt 24): 4901–13. doi:10.1242/jcs.00219. PMID 12432077. 
• Jantsch-Plunger V, Gönczy P, Romano A, Schnabel H, Hamill D, Schnabel R et al. (2000). "CYK-4: A Rho family gtpase activating protein (GAP) required for central spindle formation and cytokinesis.". J Cell Biol 149 (7): 1391–404. doi:10.1083/jcb.149.7.1391. PMC 2175131. PMID 10871280. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2175131.