RAD17

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RAD17 homolog (S. pombe)
Identifiers
Symbols RAD17 ; CCYC; HRAD17; R24L; RAD17SP; RAD24
External IDs OMIM603139 MGI1333807 HomoloGene32117 GeneCards: RAD17 Gene
RNA expression pattern
PBB GE RAD17 207405 s at tn.png
PBB GE RAD17 210826 x at tn.png
PBB GE RAD17 211228 s at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 5884 19356
Ensembl ENSG00000152942 ENSMUSG00000021635
UniProt O75943 Q6NXW6
RefSeq (mRNA) NM_001278622 NM_001044371
RefSeq (protein) NP_001265551 NP_001037836
Location (UCSC) Chr 5:
68.67 – 68.71 Mb
Chr 13:
100.62 – 100.65 Mb
PubMed search [1] [2]

Cell cycle checkpoint protein RAD17 is a protein that in humans is encoded by the RAD17 gene.[1][2]

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported.[3]

Interactions[edit]

RAD17 has been shown to interact with RAD9A,[4][5][6][7] RAD1 homolog,[5][6][8] POLE,[9] NHP2L1,[10] Ataxia telangiectasia mutated,[8][11] HUS1[5][6] and Ataxia telangiectasia and Rad3 related.[8][11]

References[edit]

  1. ^ Bao S, Shen X, Shen K, Liu Y, Wang XF (March 1999). "The mammalian Rad24 homologous to yeast Saccharomyces cerevisiae Rad24 and Schizosaccharomyces pombe Rad17 is involved in DNA damage checkpoint". Cell Growth Differ 9 (12): 961–7. PMID 9869296. 
  2. ^ Parker AE, Van de Weyer I, Laus MC, Verhasselt P, Luyten WH (August 1998). "Identification of a human homologue of the Schizosaccharomyces pombe rad17+ checkpoint gene". J Biol Chem 273 (29): 18340–6. doi:10.1074/jbc.273.29.18340. PMID 9660800. 
  3. ^ "Entrez Gene: RAD17 RAD17 homolog (S. pombe)". 
  4. ^ Dufault, Vanessa M; Oestreich Andrea J; Vroman Benjamin T; Karnitz Larry M (Dec 2003). "Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene". Genomics (United States) 82 (6): 644–51. doi:10.1016/S0888-7543(03)00200-3. ISSN 0888-7543. PMID 14611806. 
  5. ^ a b c Bermudez, Vladimir P; Lindsey-Boltz Laura A, Cesare Anthony J, Maniwa Yoshimasa, Griffith Jack D, Hurwitz Jerard, Sancar Aziz (February 2003). "Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro". Proc. Natl. Acad. Sci. U.S.A. (United States) 100 (4): 1633–8. doi:10.1073/pnas.0437927100. ISSN 0027-8424. PMC 149884. PMID 12578958. 
  6. ^ a b c Rauen, M; Burtelow M A; Dufault V M; Karnitz L M (September 2000). "The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9". J. Biol. Chem. (UNITED STATES) 275 (38): 29767–71. doi:10.1074/jbc.M005782200. ISSN 0021-9258. PMID 10884395. 
  7. ^ Lindsey-Boltz, L A; Bermudez V P; Hurwitz J; Sancar A (September 2001). "Purification and characterization of human DNA damage checkpoint Rad complexes". Proc. Natl. Acad. Sci. U.S.A. (United States) 98 (20): 11236–41. doi:10.1073/pnas.201373498. ISSN 0027-8424. PMC 58713. PMID 11572977. 
  8. ^ a b c Bao, S; Tibbetts R S; Brumbaugh K M; Fang Y; Richardson D A; Ali A; Chen S M; Abraham R T; Wang X F (June 2001). "ATR/ATM-mediated phosphorylation of human Rad17 is required for genotoxic stress responses". Nature (England) 411 (6840): 969–74. doi:10.1038/35082110. ISSN 0028-0836. PMID 11418864. 
  9. ^ Post, Sean M; Tomkinson Alan E, Lee Eva Y-H P (October 2003). "The human checkpoint Rad protein Rad17 is chromatin-associated throughout the cell cycle, localizes to DNA replication sites, and interacts with DNA polymerase ε". Nucleic Acids Res. (England) 31 (19): 5568–75. doi:10.1093/nar/gkg765. PMC 206465. PMID 14500819. 
  10. ^ Chang, M S; Sasaki H, Campbell M S, Kraeft S K, Sutherland R, Yang C Y, Liu Y, Auclair D, Hao L, Sonoda H, Ferland L H, Chen L B (Dec 1999). "HRad17 colocalizes with NHP2L1 in the nucleolus and redistributes after UV irradiation". J. Biol. Chem. (UNITED STATES) 274 (51): 36544–9. doi:10.1074/jbc.274.51.36544. ISSN 0021-9258. PMID 10593953. 
  11. ^ a b Kim, S T; Lim D S; Canman C E; Kastan M B (Dec 1999). "Substrate specificities and identification of putative substrates of ATM kinase family members". J. Biol. Chem. (UNITED STATES) 274 (53): 37538–43. doi:10.1074/jbc.274.53.37538. ISSN 0021-9258. PMID 10608806. 

Further reading[edit]