DNA repair protein REV1 is a protein that in humans is encoded by the REV1gene. This gene encodes a protein with similarity to the S. cerevisiae mutagenesis protein Rev1. The Rev1 proteins contain a BRCT domain, which is important in protein-protein interactions. A suggested role for the human Rev1-like protein is as a scaffold that recruits DNA polymerases involved in translesion synthesis (TLS) of damaged DNA. Two alternatively spliced transcript variants that encode different proteins have been found. Rev1 is a Y family DNA polymerase; it is sometimes referred to as a deoxycytidyl transferase because it only inserts deoxycytidine (dC) across from lesions. Whether G, A, T, C, or an abasic site, Rev1 will always add a C. Rev1 has the ability to always add a C, because it uses an arginine as a template which complements well with C. Yet it is believed[by whom?] that Rev1 rarely uses its polymerase activity, rather it is thought that Rev1's primary role is as a protein landing pad, whereby it helps direct the recruitment of TLS proteins, especially Pol ζ (Rev3/Rev7).
REV1 has been shown to interact with MAD2L2. It is believed that Rev1 may interact with PCNA, once ubiquitylated due to a lesion, and help recruit Pol ζ (Rev3/Rev7) a B family polymerase involved in TLS.
^Murakumo, Y; Ogura Y, Ishii H, Numata S, Ichihara M, Croce C M, Fishel R, Takahashi M (September 2001). "Interactions in the error-prone postreplication repair proteins hREV1, hREV3, and hREV7". J. Biol. Chem. (United States) 276 (38): 35644–51. doi:10.1074/jbc.M102051200. ISSN0021-9258. PMID11485998.Cite uses deprecated parameters (help)
Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery.". Genome Res.6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID8889548.
Wixler V, Laplantine E, Geerts D, et al. (1999). "Identification of novel interaction partners for the conserved membrane proximal region of alpha-integrin cytoplasmic domains.". FEBS Lett.445 (2-3): 351–5. doi:10.1016/S0014-5793(99)00151-9. PMID10094488.
Wixler V, Geerts D, Laplantine E, et al. (2000). "The LIM-only protein DRAL/FHL2 binds to the cytoplasmic domain of several alpha and beta integrin chains and is recruited to adhesion complexes.". J. Biol. Chem.275 (43): 33669–78. doi:10.1074/jbc.M002519200. PMID10906324.
Masuda Y, Takahashi M, Tsunekuni N, et al. (2001). "Deoxycytidyl transferase activity of the human REV1 protein is closely associated with the conserved polymerase domain.". J. Biol. Chem.276 (18): 15051–8. doi:10.1074/jbc.M008082200. PMID11278384.
Murakumo Y, Ogura Y, Ishii H, et al. (2001). "Interactions in the error-prone postreplication repair proteins hREV1, hREV3, and hREV7.". J. Biol. Chem.276 (38): 35644–51. doi:10.1074/jbc.M102051200. PMID11485998.
Masuda Y, Ohmae M, Masuda K, Kamiya K (2003). "Structure and enzymatic properties of a stable complex of the human REV1 and REV7 proteins.". J. Biol. Chem.278 (14): 12356–60. doi:10.1074/jbc.M211765200. PMID12529368.
Tissier A, Kannouche P, Reck MP, et al. (2005). "Co-localization in replication foci and interaction of human Y-family members, DNA polymerase pol eta and REVl protein.". DNA Repair (Amst.)3 (11): 1503–14. doi:10.1016/j.dnarep.2004.06.015. PMID15380106.
Hillier LW, Graves TA, Fulton RS, et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4.". Nature434 (7034): 724–31. doi:10.1038/nature03466. PMID15815621.
Lin X, Okuda T, Trang J, Howell SB (2006). "Human REV1 modulates the cytotoxicity and mutagenicity of cisplatin in human ovarian carcinoma cells.". Mol. Pharmacol.69 (5): 1748–54. doi:10.1124/mol.105.020446. PMID16495473.
Yuasa MS, Masutani C, Hirano A, et al. (2006). "A human DNA polymerase eta complex containing Rad18, Rad6 and Rev1; proteomic analysis and targeting of the complex to the chromatin-bound fraction of cells undergoing replication fork arrest.". Genes Cells11 (7): 731–44. doi:10.1111/j.1365-2443.2006.00974.x. PMID16824193.