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Replication factor C (activator 1) 2, 40kDa
Symbols RFC2 ; RFC40
External IDs OMIM600404 MGI1341868 HomoloGene6885 GeneCards: RFC2 Gene
RNA expression pattern
PBB GE RFC2 1053 at tn.png
PBB GE RFC2 203696 s at tn.png
More reference expression data
Species Human Mouse
Entrez 5982 19718
Ensembl ENSG00000049541 ENSMUSG00000023104
UniProt P35250 Q9WUK4
RefSeq (mRNA) NM_001278791 NM_020022
RefSeq (protein) NP_001265720 NP_064406
Location (UCSC) Chr 7:
73.65 – 73.67 Mb
Chr 5:
134.58 – 134.6 Mb
PubMed search [1] [2]

Replication factor C subunit 2 is a protein that in humans is encoded by the RFC2 gene.[1][2][3]

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described.[3]


RFC2 has been shown to interact with BRD4,[4] CHTF18,[5][6] PCNA,[7][8][9] RFC4[7][10] and RFC5.[7][10]


  1. ^ Chen M, Pan ZQ, Hurwitz J (May 1992). "Sequence and expression in Escherichia coli of the 40-kDa subunit of activator 1 (replication factor C) of HeLa cells". Proc Natl Acad Sci U S A 89 (7): 2516–20. doi:10.1073/pnas.89.7.2516. PMC 48692. PMID 1313560. 
  2. ^ Okumura K, Nogami M, Taguchi H, Dean FB, Chen M, Pan ZQ, Hurwitz J, Shiratori A, Murakami Y, Ozawa K et al. (Jul 1995). "Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23". Genomics 25 (1): 274–8. doi:10.1016/0888-7543(95)80135-9. PMID 7774928. 
  3. ^ a b "Entrez Gene: RFC2 replication factor C (activator 1) 2, 40kDa". 
  4. ^ Maruyama, Tetsuo; Farina Andrea; Dey Anup; Cheong JaeHun; Bermudez Vladimir P; Tamura Tomohiko; Sciortino Selvaggia; Shuman Jon; Hurwitz Jerard; Ozato Keiko (Sep 2002). "A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase". Mol. Cell. Biol. (United States) 22 (18): 6509–20. doi:10.1128/MCB.22.18.6509-6520.2002. ISSN 0270-7306. PMC 135621. PMID 12192049. 
  5. ^ Bermudez, Vladimir P; Maniwa Yoshimasa; Tappin Inger; Ozato Keiko; Yokomori Kyoko; Hurwitz Jerard (Sep 2003). "The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA". Proc. Natl. Acad. Sci. U.S.A. (United States) 100 (18): 10237–42. doi:10.1073/pnas.1434308100. ISSN 0027-8424. PMC 193545. PMID 12930902. 
  6. ^ Merkle, Carolin J; Karnitz Larry M, Henry-Sánchez John T, Chen Junjie (Aug 2003). "Cloning and characterization of hCTF18, hCTF8, and hDCC1. Human homologs of a Saccharomyces cerevisiae complex involved in sister chromatid cohesion establishment". J. Biol. Chem. (United States) 278 (32): 30051–6. doi:10.1074/jbc.M211591200. ISSN 0021-9258. PMID 12766176. 
  7. ^ a b c Cai, J; Gibbs E; Uhlmann F; Phillips B; Yao N; O'Donnell M; Hurwitz J (Jul 1997). "A complex consisting of human replication factor C p40, p37, and p36 subunits is a DNA-dependent ATPase and an intermediate in the assembly of the holoenzyme". J. Biol. Chem. (UNITED STATES) 272 (30): 18974–81. doi:10.1074/jbc.272.30.18974. ISSN 0021-9258. PMID 9228079. 
  8. ^ Pan, Z Q; Chen M; Hurwitz J (Jan 1993). "The subunits of activator 1 (replication factor C) carry out multiple functions essential for proliferating-cell nuclear antigen-dependent DNA synthesis". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 90 (1): 6–10. doi:10.1073/pnas.90.1.6. ISSN 0027-8424. PMC 45588. PMID 8093561. 
  9. ^ Ohta, Satoshi; Shiomi Yasushi; Sugimoto Katsunori; Obuse Chikashi; Tsurimoto Toshiki (Oct 2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". J. Biol. Chem. (United States) 277 (43): 40362–7. doi:10.1074/jbc.M206194200. ISSN 0021-9258. PMID 12171929. 
  10. ^ a b Cai, J; Yao N; Gibbs E; Finkelstein J; Phillips B; O'Donnell M; Hurwitz J (Sep 1998). "ATP hydrolysis catalyzed by human replication factor C requires participation of multiple subunits". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 95 (20): 11607–12. doi:10.1073/pnas.95.20.11607. ISSN 0027-8424. PMC 21688. PMID 9751713. 

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