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Replication factor C (activator 1) 3, 38kDa
Symbols RFC3 ; RFC38
External IDs OMIM600405 MGI1916513 HomoloGene2188 GeneCards: RFC3 Gene
RNA expression pattern
PBB GE RFC3 204127 at tn.png
PBB GE RFC3 204128 s at tn.png
More reference expression data
Species Human Mouse
Entrez 5983 69263
Ensembl ENSG00000133119 ENSMUSG00000033970
UniProt P40938 Q8R323
RefSeq (mRNA) NM_002915 NM_027009
RefSeq (protein) NP_002906 NP_081285
Location (UCSC) Chr 13:
34.39 – 34.54 Mb
Chr 5:
151.64 – 151.65 Mb
PubMed search [1] [2]

Replication factor C subunit 3 is a protein that in humans is encoded by the RFC3 gene.[1][2]

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.[2]


RFC3 has been shown to interact with BRD4,[3] CHTF18,[4][5] RFC1,[6][7][8] PCNA[5][9] and RFC4.[6][7]


  1. ^ Okumura K, Nogami M, Taguchi H, Dean FB, Chen M, Pan ZQ, Hurwitz J, Shiratori A, Murakami Y, Ozawa K, et al. (Jul 1995). "Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23". Genomics 25 (1): 274–8. doi:10.1016/0888-7543(95)80135-9. PMID 7774928. 
  2. ^ a b "Entrez Gene: RFC3 replication factor C (activator 1) 3, 38kDa". 
  3. ^ Maruyama, Tetsuo; Farina Andrea; Dey Anup; Cheong JaeHun; Bermudez Vladimir P; Tamura Tomohiko; Sciortino Selvaggia; Shuman Jon; Hurwitz Jerard; Ozato Keiko (Sep 2002). "A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase". Mol. Cell. Biol. (United States) 22 (18): 6509–20. doi:10.1128/MCB.22.18.6509-6520.2002. ISSN 0270-7306. PMC 135621. PMID 12192049. 
  4. ^ Bermudez, Vladimir P; Maniwa Yoshimasa; Tappin Inger; Ozato Keiko; Yokomori Kyoko; Hurwitz Jerard (Sep 2003). "The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA". Proc. Natl. Acad. Sci. U.S.A. (United States) 100 (18): 10237–42. doi:10.1073/pnas.1434308100. ISSN 0027-8424. PMC 193545. PMID 12930902. 
  5. ^ a b Merkle, Carolin J; Karnitz Larry M, Henry-Sánchez John T, Chen Junjie (Aug 2003). "Cloning and characterization of hCTF18, hCTF8, and hDCC1. Human homologs of a Saccharomyces cerevisiae complex involved in sister chromatid cohesion establishment". J. Biol. Chem. (United States) 278 (32): 30051–6. doi:10.1074/jbc.M211591200. ISSN 0021-9258. PMID 12766176. 
  6. ^ a b Ellison, V; Stillman B (Mar 1998). "Reconstitution of recombinant human replication factor C (RFC) and identification of an RFC subcomplex possessing DNA-dependent ATPase activity". J. Biol. Chem. (UNITED STATES) 273 (10): 5979–87. doi:10.1074/jbc.273.10.5979. ISSN 0021-9258. PMID 9488738. 
  7. ^ a b Uhlmann, F; Cai J; Flores-Rozas H; Dean F B; Finkelstein J; O'Donnell M; Hurwitz J (Jun 1996). "In vitro reconstitution of human replication factor C from its five subunits". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 93 (13): 6521–6. doi:10.1073/pnas.93.13.6521. ISSN 0027-8424. PMC 39056. PMID 8692848. 
  8. ^ Tomida, Junya; Masuda Yuji, Hiroaki Hidekazu, Ishikawa Tomoko, Song Ihnyoung, Tsurimoto Toshiki, Tateishi Satoshi, Shiomi Tadahiro, Kamei Yasuhiro, Kim Jinhyeong, Kamiya Kenji, Vaziri Cyrus, Ohmori Haruo, Todo Takeshi (Apr 2008). "DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex". J. Biol. Chem. (United States) 283 (14): 9071–9. doi:10.1074/jbc.M709835200. ISSN 0021-9258. PMC 2431014. PMID 18245774. 
  9. ^ Ohta, Satoshi; Shiomi Yasushi; Sugimoto Katsunori; Obuse Chikashi; Tsurimoto Toshiki (Oct 2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". J. Biol. Chem. (United States) 277 (43): 40362–7. doi:10.1074/jbc.M206194200. ISSN 0021-9258. PMID 12171929. 

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