RNA extraction is the purification of RNA from biological samples. This procedure is complicated by the ubiquitous presence of ribonuclease enzymes in cells and tissues, which can rapidly degrade RNA. Several methods are used in molecular biology to isolate RNA from samples, the most common of these is Guanidinium thiocyanate-phenol-chloroform extraction.
RNA extraction in liquid nitrogen, commonly using a mortar and pestle, or specialised motorized grinding device cell lysis device is also useful in preventing ribonuclease activity.
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