RNA spike-in

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A RNA spike-in is a RNA transcript used to calibrate measurements in a DNA microarray experiment. Each spike-in is designed to hybridize with a specific control probe on the target array. Manufacturers of commercially available microarrays typically offer companion RNA spike-ins "kits".[1][2][3][4]

Known amounts of RNA spike-ins are mixed with the experiment sample during preparation. Subsequently the measured degree of hybridization between the spike-ins and the control probes is used to normalize the hybridization measurements of the sample RNA.

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References[edit]

  1. ^ Yang IV (2006). "Use of external controls in microarray experiments.". Methods Enzymol 411: 50–63. doi:10.1016/S0076-6879(06)11004-6. PMID 16939785. 
  2. ^ Fardin P, Moretti S, Biasotti B, Ricciardi A, Bonassi S, Varesio L (2007). "Normalization of low-density microarray using external spike-in controls: analysis of macrophage cell lines expression profile.". BMC Genomics 8: 17. doi:10.1186/1471-2164-8-17. PMC 1797020. PMID 17229315. 
  3. ^ Wilkes T, Laux H, Foy CA (2007). "Microarray data quality - review of current developments.". OMICS 11 (1): 1–13. doi:10.1089/omi.2006.0001. PMID 17411392. 
  4. ^ Schuster EF, Blanc E, Partridge L, Thornton JM (2007). "Estimation and correction of non-specific binding in a large-scale spike-in experiment.". Genome Biol 8 (6): R126. doi:10.1186/gb-2007-8-6-r126. PMC 2394775. PMID 17594493.