Raffaele Marcellino graduated from the Sydney Conservatorium of Music with merit in 1985. His teachers included Richard Vella, Richard Toop, Gillian Whitehead and Božidar Kos (sl). In 1995 Marcellino joined the staff of the Tasmanian Conservatorium of Music where he served as Director from 1996–1998 and resumed normal teaching duties in 1999. In 1999 Arts Tasmania funded project the Mountain Orchestra Project, a community arts project with Marcellino was composer and music director and Strato Anagnostis, instrument maker and performer. The Mountain Orchestra was made up of community members who constructed instruments from found objects and other materials and then performed newly composed in a concert on Mt Wellington in Hobart. During his time in Tasmania also served on the Board of the Inaugural '10 Days on the Island Festival' and 'Zootango'.
At the end of 2001 he left the University of Tasmania and returned to Sydney to pursue a freelance career and sessional teaching at the Sydney Conservatorium of Music. In 2003 Marcellino was Composer-in-Residence with eminent Australian vocal ensemble the Song Company, who had previously commissioned several pieces from him. This residency culminated in the choral cycle The O Antiphons and released on CD, widely performed and broadcast. From 2003 until 2009 Marcellino was the Principal of the Australian Institute of Music in Sydney. In 2010 he became Foundation Dean of the Australian College of the Arts Collarts in Melbourne. After establishing Collarts as a degree granting institution he was appointed Dean of Macleay College  in Sydney in 2011. In 2013 he was appointed Director of Academic and Student Services for SAE Australia. Recent creative collaborations have included prominent Australian artists including Greg White (composer), Robert Jarman(actor and theatre maker), Anna Messariti (producer and theatre maker), The Song Company, Sirens Ensemble, Tom O'Kelly (percussionist), Michael Bates (filmmaker) and Jordie Albiston (poet).
- Responsorio — for SATB choir; premiered in Sydney by The Contemporary Singers in 1988 , also performed by the Sydney Chamber Choir.
- Remedy — an opera in one act; libretto by Marguerite Bunce.
- FishTale — for chamber choir, performed by The Song Company.
- Logos — for choir and digital delay, performed by Sydney Chamber Choir.
- Thirst — with Greg White at the Australian Museum for director Yaron Lifschitz and designer Genevieve Blanchett
- The Art of Resonance — tuba concerto, performed by Steve Ross and the Sydney Symphony Orchestra
- On Eagles Wings — at the International Tuba Conference University of Minnesota
- Zerfliesse mein Herze — performed by Ian Munro, Christian Wojtowicz, Tom O'Kelly and David Malone[disambiguation needed] and featured in A Fork in the Road episode featuring Tasmania
- Voicejam and Video — at the Adelaide Festival
- Canticle — for singers, actors and large ensemble premiered at the Brisbane Cathedrals Festival.
- Musica Viva — performed by Tom O'Kelly (Percussion) in Japan.
- November: Bach: a sonic exhibition — co-curated with Greg White, performed by the Seymour Group.
- Heart of Fire — with Greg White; commissioned by ABC Sport as the theme for the 2000 Paralympics; released as a CD single on ABC Classics.
- September: Maze for 15 musicians — performed by Ensemble Modern during the International Society of Contemporary Music World Music Days in Luxembourg.
- Sprung! — performed by The Song Company.
- The Flight of Les Darcy — an opera, premiered at the 10 Days on the Island festival, Hobart, Tasmania. Libretto by Robert Jarman.
- November: The Dædalus Sequences — cycle for solo piano, performed by Ian Munro in Newcastle-upon-Tyne.
- November: The Pluperfect Square Dances — performed by Tom O'Kelly in Kunazawa, Japan.
- August: The Art of Memory — performed by Marina Phillips and the Australian Brandenburg Orchestra at the City Recital Hall, Sydney.
- L'arte di volare — performed by the Tasmanian Symphony Orchestra
- The Art of Perception — performed by the Australia Ensemble at the International Music and Psychology Conference, Sydney
- Q — performed by David Malone[disambiguation needed] at the Darwin International Guitar Festival
- Clarion Call, — a site specific work for the Queensland Conservatorium Griffith University.
- Mrs Macquarie's Cello — a music theatre work for The Song Company.
- Midnite — performed by the Australian Opera at Melbourne Festival.
- Universal Beatitudes — an International collaborative song cycle.
- Ein Psalm Davids — SATB choir and solo violin, performed by the Sydney Philharmonia Motet Choir and Rachel Beesley as part of the Immortal Bach series at the Eugene Goosens Hall, ABC Centre Ultimo.
- Dinner Fanfare — brass ensemble performed by the Sydney Youth Orchestra Brass at the Sydney Children's Hospital 2007 Gold Dinner Charity Event.
- Remembrance of Things Past — solo viola and chamber orchestra performed by Marina Phillips and the Sydney Youth Orchestra Brass at the Australian Institute of Music.
- Going Postal — trumpet and percussion commissioned by Tom O'Kelly.
- Hekuba's Lament — voice and chamber ensemble commissioned by Much Ado Productions.
- War is not the Season for Figs — voice, accordion and cello commissioned and broadcast on ABC Radio National on its Poetica program featuring the poetry of Linda Cvetkovic.
Marcellino's music has been published by Reed Music, Currency Press, Opus House Press, Red House Editions, Grevillea Press and ABC Classics.
- Music Fellowship awarded by the Music Board of the Australia Council (April 2003)
- Paul Lowin Song Cycle Prize (1999), First Prize for Canticle
- Paul Lowin Song Cycle Prize (1997), Highly Commended for FishTale