She graduated from law school in 1999 and in 2001 started her work as lawyer. She has been a member of the team of lawyers for defense of political prisoners since 2001. In the same year, Razan was one of the founders of the Human Rights Association in Syria (HRAS). In 2005, Razan Zaitouneh established SHRIL (the Syrian Human Rights Information Link), through which she continues to report about human rights violations in Syria. Since 2005, Razan Zaitouneh is also an active member of the Committee to Support Families of Political Prisoners in Syria.
In December 2013, pro-opposition websites reported that Zaitouneh was kidnapped along with her husband and two colleagues in the opposition-held town of Douma to the north of Damascus. As of 4 February 2014, their whereabouts were still unknown and the identity of the kidnappers uncertain, although it was suspected that the Islamist rebel group the Army of Islam was responsible.