Reactive nitrogen species
Reactive nitrogen species (RNS) are a family of antimicrobial molecules derived from nitric oxide (·NO) and superoxide (O2·−) produced via the enzymatic activity of inducible nitric oxide synthase 2 (NOS2) and NADPH oxidase respectively. NOS2 is expressed primarily in macrophages after induction by cytokines and microbial products, notably interferon-gamma (IFN-γ) and lipopolysaccharide (LPS).
- ·NO (nitric oxide) + O2·− (superoxide) → ONOO− (peroxynitrite)
Superoxide anion (O2−) is a reactive oxygen species that reacts quickly with nitric oxide (NO) in the vasculature. The reaction produces peroxynitrite and depletes the bioactivity of NO. This is important because NO is a key mediator in many important vascular functions including regulation of smooth muscle tone and blood pressure, platelet activation, and vascular cell signaling.
Peroxynitrite itself is a highly reactive species which can directly react with various biological targets and components of the cell including lipids, thiols, amino acid residues, DNA bases, and low-molecular weight antioxidants. However, these reactions happen at a relatively slow rate. This slow reaction rate allows it to react more selectively throughout the cell. Peroxynitrite is able to get across cell membranes to some extent through anion channels. Additionally peroxynitrite can react with other molecules to form additional types of RNS including nitrogen dioxide (·NO2) and dinitrogen trioxide (N2O3) as well as other types of chemically reactive free radicals. Important reactions involving RNS include:
- ONOO− + H+ → ONOOH (peroxynitrous acid) → ·NO2 (nitrogen dioxide) + ·OH (hydroxyl radical)
- ONOO− + CO2 (carbon dioxide) → ONOOCO2− (nitrosoperoxycarbonate)
- ONOOCO2− → ·NO2 (nitrogen dioxide) + O=C(O·)O− (carbonate radical)
- ·NO + ·NO2 N2O3 (dinitrogen trioxide)
Peroxynitrite can react directly with proteins that contain transition metal centers. Therefore, it can modify proteins such as hemoglobin, myoglobin, and cytochrome c by oxidizing ferrous heme into its corresponding ferric forms. Peroxynitrite may also be able to change protein structure through the reaction with various amino acids in the peptide chain. The most common reaction with amino acids is cysteine oxidation. Another reaction is tyrosine nitration; however peroxynitrite does not react directly with tyrosine. Tyrosine reacts with other RNS that are produced by peroxynitrite. All of these reactions affect protein structure and function and thus have the potential to cause changes in the catalytic activity of enzymes, altered cytoskeletal organization, and impaired cell signal transduction.
- Novo E, Parola M (2008). "Redox mechanisms in hepatic chronic wound healing and fibrogenesis". Fibrogenesis Tissue Repair 1 (1): 5. doi:10.1186/1755-1536-1-5. PMC 2584013. PMID 19014652.
- Iovine N, Pursnani S, Voldman A, Wasserman G, Blaser M, Weinrauch Y (March 2008). "Reactive nitrogen species contribute to innate host defense against Campylobacter jejuni". Infection and Immunity 76 (3): 986–93. doi:10.1128/IAI.01063-07. PMC 2258852. PMID 18174337.
- Pauly N, Pucciariello C, Mandon K, Innocenti G, Jamet A, Baudouin E et al. (2006). "Reactive oxygen and nitrogen species and glutathione: key players in the legume-Rhizobium symbiosis". Journal of Experimental Botany 57 (8): 1769–76. doi:10.1093/jxb/erj184. PMID 16698817.
- Squadrito G, Pryor W (September 1998). "Oxidative chemistry of nitric oxide: the roles of superoxide, peroxynitrite, and carbon dioxide". Free Radical Biology and Medicine 25 (4–5): 392–403. doi:10.1016/S0891-5849(98)00095-1. PMID 9741578.
- Dröge W (January 2002). "Free radicals in the physiological control of cell function". Physiological Reviews 82 (1): 47–95. doi:10.1152/physrev.00018.2001 (inactive 2015-01-01). PMID 11773609.
- Guzik T, West N, Pillai R, Taggart D, Channon K (June 2002). "Nitric oxide modulates superoxide release and peroxynitrite formation in human blood vessels". Hypertension 39 (6): 1088–94. doi:10.1161/01.HYP.0000018041.48432.B5. PMID 12052847.
- O'Donnell V, Eiserich J, Chumley P, Jablonsky M, Krishna N, Kirk M et al. (January 1999). "Nitration of unsaturated fatty acids by nitric oxide-derived reactive nitrogen species peroxynitrite, nitrous acid, nitrogen dioxide, and nitronium ion". Chem. Res. Toxicol. 12 (1): 83–92. doi:10.1021/tx980207u. PMID 9894022.
- Pacher P, Beckman J, Liaudet L (January 2007). "Nitric oxide and peroxynitrite in health and disease". Physiol. Rev. 87 (1): 315–424. doi:10.1152/physrev.00029.2006. PMC 2248324. PMID 17237348.