Renilla-luciferin 2-monooxygenase

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Renilla-luciferin 2-monooxygenase
Renilla reniformis luciferase with bound coelenterazine. PDB 2pdj [1]
EC number
CAS number 61869-41-8
IntEnz IntEnz view
ExPASy NiceZyme view
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO

Renilla-luciferin 2-monooxygenase (EC is an enzyme that catalyzes the chemical reaction

Renilla luciferin + O2 \rightleftharpoons oxidized Renilla luciferin + CO2 + hν

The two substrates of this enzyme are Coelenterazine and O2, whereas its 3 products are coelenteramide, CO2, and a photon of blue light.

This enzyme was isolated from Renilla reniformis, the sea pansy, a bioluminescent sea pen. The enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O2 as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). The oxygen incorporated need not be derived from O with incorporation of one atom of oxygen (internal monooxygenases o internal mixed-function oxidases).

In vivo, the photon emitted by Renilla-luciferin 2-monooxygenase transferred to green fluorescent protein, causing it to emit green light.

The systematic name of this enzyme class is Renilla-luciferin:oxygen 2-oxidoreductase (decarboxylating). Other names in common use include Renilla-type luciferase, aequorin, and luciferase (Renilla luciferin).


  1. ^ Loening, A. M.; Fenn, T. D.; Gambhir, S. S. (2007). "Crystal Structures of the Luciferase and Green Fluorescent Protein from Renilla reniformis". Journal of Molecular Biology 374 (4): 1017–1028. doi:10.1016/j.jmb.2007.09.078. PMC 2700051. PMID 17980388.  edit