Replication protein A

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Replication protein A C terminal
Identifiers
Symbol RPA_C
Pfam PF08784
InterPro IPR014892

Replication protein A (RPA) is a protein that binds to single-stranded DNA in eukaryotic cells.[1] During DNA replication, RPA prevents single-stranded DNA (ssDNA) from winding back on itself or from forming secondary structures. This keeps DNA unwound for the polymerase to replicate it. RPA also binds to ssDNA during the initial phase of homologous recombination, an important process in DNA repair and prophase I of meiosis. Like its role in DNA replication, this keeps ssDNA from binding to itself (self-complementizing) so that the resulting nucleoprotein filament can then bound by Rad51 and its cofactors.[2] RPA also binds to DNA during the Nucleotide Excision Repair process. This binding stabilizes the repair complex during the repair process. A bacterial homolog is called single-strand binding protein (SSB).

References[edit]

  1. ^ Wold, MS (1997). "Replication protein A: heterotrimeric, single-stranded DNA-binding protein required for eukaryotic DNA metabolism". Annual Review of Biochemistry 66 (1): 61–92. doi:10.1146/annurev.biochem.66.1.61. PMID 9242902. 
  2. ^ Xuan, L; Wolf-Dietrich, H (2008). "Homologous recombination in DNA repair and DNA damage tolerance". Cell Research 18 (99): 99–113. doi:10.1038/cr.2008.1. PMC 3087377. PMID 18166982.