Replication protein A1

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Replication protein A1, 70kDa
Protein RPA1 PDB 1ewi.png
PDB rendering based on 1ewi.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols RPA1 ; HSSB; MST075; REPA1; RF-A; RP-A; RPA70
External IDs OMIM179835 MGI1915525 HomoloGene2208 ChEMBL: 1764940 GeneCards: RPA1 Gene
Species Human Mouse
Entrez 6117 68275
Ensembl ENSG00000132383 ENSMUSG00000000751
UniProt P27694 Q8VEE4
RefSeq (mRNA) NM_002945 NM_001164223
RefSeq (protein) NP_002936 NP_001157695
Location (UCSC) Chr 17:
1.73 – 1.8 Mb
Chr 11:
75.3 – 75.35 Mb
PubMed search [1] [2]

Replication protein A 70 kDa DNA-binding subunit is a protein that in humans is encoded by the RPA1 gene.[1]


Replication protein A1 has been shown to interact with RPA2,[2][3] RPA3,[3][4] MCM6,[5] MCM7,[5] MUTYH,[6] BRCA2,[7] ORC6L,[5] ORC2L,[5] TP53BP1,[8] MCM4,[5] P53,[9][10] XPA,[11] MCM2,[5] TIPIN[12] and Bloom syndrome protein.[13][14][15]


  1. ^ Umbricht CB, Erdile LF, Jabs EW, Kelly TJ (April 1993). "Cloning, overexpression, and genomic mapping of the 14-kDa subunit of human replication protein A". J Biol Chem 268 (9): 6131–8. PMID 8454588. 
  2. ^ Shao, R G; Cao C X; Zhang H; Kohn K W; Wold M S; Pommier Y (March 1999). "Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes". EMBO J. (ENGLAND) 18 (5): 1397–406. doi:10.1093/emboj/18.5.1397. ISSN 0261-4189. PMC 1171229. PMID 10064605. 
  3. ^ a b Bochkareva, Elena; Korolev Sergey, Lees-Miller Susan P, Bochkarev Alexey (April 2002). "Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA". EMBO J. (England) 21 (7): 1855–63. doi:10.1093/emboj/21.7.1855. ISSN 0261-4189. PMC 125950. PMID 11927569. 
  4. ^ Bochkareva, E; Frappier L; Edwards A M; Bochkarev A (February 1998). "The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain". J. Biol. Chem. (UNITED STATES) 273 (7): 3932–6. doi:10.1074/jbc.273.7.3932. ISSN 0021-9258. PMID 9461578. 
  5. ^ a b c d e f Kneissl, Margot; Pütter Vera; Szalay Aladar A; Grummt Friedrich (March 2003). "Interaction and assembly of murine pre-replicative complex proteins in yeast and mouse cells". J. Mol. Biol. (England) 327 (1): 111–28. doi:10.1016/S0022-2836(03)00079-2. ISSN 0022-2836. PMID 12614612. 
  6. ^ Parker, A; Gu Y; Mahoney W; Lee S H; Singh K K; Lu A L (February 2001). "Human homolog of the MutY repair protein (hMYH) physically interacts with proteins involved in long patch DNA base excision repair". J. Biol. Chem. (United States) 276 (8): 5547–55. doi:10.1074/jbc.M008463200. ISSN 0021-9258. PMID 11092888. 
  7. ^ Wong, Johnson M S; Ionescu Daniela; Ingles C James (January 2003). "Interaction between BRCA2 and replication protein A is compromised by a cancer-predisposing mutation in BRCA2". Oncogene (England) 22 (1): 28–33. doi:10.1038/sj.onc.1206071. ISSN 0950-9232. PMID 12527904. 
  8. ^ Yoo, Eunjae; Kim Byung U; Lee Seung Youn; Cho Chae Hyun; Chung Jay H; Lee Chang-Hun (August 2005). "53BP1 is associated with replication protein A and is required for RPA2 hyperphosphorylation following DNA damage". Oncogene (England) 24 (35): 5423–30. doi:10.1038/sj.onc.1208710. ISSN 0950-9232. PMID 15856006. 
  9. ^ Romanova, Larisa Y; Willers Henning; Blagosklonny Mikhail V; Powell Simon N (Dec 2004). "The interaction of p53 with replication protein A mediates suppression of homologous recombination". Oncogene (England) 23 (56): 9025–33. doi:10.1038/sj.onc.1207982. ISSN 0950-9232. PMID 15489903. 
  10. ^ Riva, F; Zuco V; Vink A A; Supino R; Prosperi E (Dec 2001). "UV-induced DNA incision and proliferating cell nuclear antigen recruitment to repair sites occur independently of p53-replication protein A interaction in p53 wild type and mutant ovarian carcinoma cells". Carcinogenesis (England) 22 (12): 1971–8. doi:10.1093/carcin/22.12.1971. ISSN 0143-3334. PMID 11751427. 
  11. ^ Li, L; Lu X; Peterson C A; Legerski R J (October 1995). "An interaction between the DNA repair factor XPA and replication protein A appears essential for nucleotide excision repair". Mol. Cell. Biol. (UNITED STATES) 15 (10): 5396–402. ISSN 0270-7306. PMC 230789. PMID 7565690. 
  12. ^ Unsal-Kaçmaz, Keziban; Chastain Paul D, Qu Ping-Ping, Minoo Parviz, Cordeiro-Stone Marila, Sancar Aziz, Kaufmann William K (April 2007). "The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement". Mol. Cell. Biol. (United States) 27 (8): 3131–42. doi:10.1128/MCB.02190-06. ISSN 0270-7306. PMC 1899931. PMID 17296725. 
  13. ^ Brosh, R M; Li J L; Kenny M K; Karow J K; Cooper M P; Kureekattil R P; Hickson I D; Bohr V A (August 2000). "Replication protein A physically interacts with the Bloom's syndrome protein and stimulates its helicase activity". J. Biol. Chem. (UNITED STATES) 275 (31): 23500–8. doi:10.1074/jbc.M001557200. ISSN 0021-9258. PMID 10825162. 
  14. ^ Opresko, Patricia L; von Kobbe Cayetano; Laine Jean-Philippe; Harrigan Jeanine; Hickson Ian D; Bohr Vilhelm A (October 2002). "Telomere-binding protein TRF2 binds to and stimulates the Werner and Bloom syndrome helicases". J. Biol. Chem. (United States) 277 (43): 41110–9. doi:10.1074/jbc.M205396200. ISSN 0021-9258. PMID 12181313. 
  15. ^ Moens, Peter B; Kolas Nadine K; Tarsounas Madalena; Marcon Edyta; Cohen Paula E; Spyropoulos Barbara (April 2002). "The time course and chromosomal localization of recombination-related proteins at meiosis in the mouse are compatible with models that can resolve the early DNA-DNA interactions without reciprocal recombination". J. Cell. Sci. (England) 115 (Pt 8): 1611–22. ISSN 0021-9533. PMID 11950880. 

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