Roxan (protein)

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Zinc finger CCCH-type containing 7B
Identifiers
Symbols ZC3H7B ; RoXaN
External IDs MGI1328310 HomoloGene9735 GeneCards: ZC3H7B Gene
RNA expression pattern
PBB GE ZC3H7B 205877 s at tn.png
PBB GE ZC3H7B 213323 s at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 23264 20286
Ensembl ENSG00000100403 ENSMUSG00000022390
UniProt Q9UGR2 F8VPP8
RefSeq (mRNA) NM_017590 NM_001081016
RefSeq (protein) NP_060060 NP_001074485
Location (UCSC) Chr 22:
41.7 – 41.76 Mb
Chr 15:
81.74 – 81.8 Mb
PubMed search [1] [2]

RoXaN (Rotavirus 'X'-associated non-structural protein) also known as ZC3H7B (zinc finger CCCH-type containing 7B), is a protein that in humans is encoded by the ZC3H7B gene.[1] RoXaN is a protein that contains tetratricopeptide repeat and leucine-aspartate repeat as well as zinc finger domains. This protein also interacts with the rotavirus non-structural protein NSP3.[1]

Function[edit]

Rotavirus mRNAs are capped but not polyadenylated, and viral proteins are translated by the cellular translation machinery. This is accomplished through the action of the viral nonstructural protein NSP3 which specifically binds the 3' consensus sequence of viral mRNAs and interacts with the eukaryotic translation initiation factor eIF4G I.[2]

RoXaN (rotavirus X protein associated with NSP3) is 110-kDa cellular protein that contains a minimum of three regions predicted to be involved in protein-protein or nucleic acid-protein interactions. A tetratricopeptide repeat region, a protein-protein interaction domain most often found in multiprotein complexes, is present in the amino-terminal region. In the carboxy terminus, at least five zinc finger motifs are observed, further suggesting the capacity of RoXaN to bind other proteins or nucleic acids. Between these two regions exists a paxillin leucine-aspartate repeat (LD) motif which is involved in protein-protein interactions.[2]

Clinical significance[edit]

RoXaN is capable of interacting with NSP3 in vivo and during rotavirus infection. Domains of interaction correspond to the dimerization domain of NSP3 (amino acids 163 to 237) and the LD domain of RoXaN (amino acids 244 to 341). The interaction between NSP3 and RoXaN does not impair the interaction between NSP3 and eIF4G I, and a ternary complex made of NSP3, RoXaN, and eIF4G I can be detected in rotavirus-infected cells, implicating RoXaN in translation regulation.[2]

Expression of RoXaN was found to be correlated with a higher tumor grad in GIST (gastrointestinal stromal tumors).[3]

References[edit]

  1. ^ a b "Entrez Gene: ZC3H7B zinc finger CCCH-type containing 7B". 
  2. ^ a b c Vitour D, Lindenbaum P, Vende P, Becker MM, Poncet D (April 2004). "RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3". J. Virol. 78 (8): 3851–62. doi:10.1128/JVI.78.8.3851-3862.2004. PMC 374268. PMID 15047801. 
  3. ^ Kang HJ, Koh KH, Yang E, You KT, Kim HJ, Paik YK, Kim H (February 2006). "Differentially expressed proteins in gastrointestinal stromal tumors with KIT and PDGFRA mutations". Proteomics 6 (4): 1151–7. doi:10.1002/pmic.200500372. PMID 16402362. 

Further reading[edit]