This article contains information on rugby league played in the UK at semi-professional level in 2007. For general information on the National Leagues, see Rugby League National Leagues. For other leagues in 2007, see Rugby league in 2007.
The league was televised live for the first time following a deal with the broadcaster Sky Sports.
Castleford Tigers were relegated from the Super League in 2006, while Dewsbury Rams and Sheffield Eagles gained promotion from National League Two. York City Knights and Oldham Roughyeds are relegated to National League Two.
National League Three was abolished for the 2007 season and rebranded as the Rugby League Conference National division.
National League One
||Team finishing top of the table, earning the League Leaders Shield
||Teams qualifying for the Play-offs
||Teams in relegation position
- Rochdale and Doncaster relegated to National League Two for 2008.
- Doncaster deducted 6 points due to entering administration.
- Whitehaven (H) beat Leigh (A) (Whitehaven through to week two, Leigh are eliminated).
- Halifax (H) beat Sheffield (A) (Halifax through to week two, Sheffield are eliminated).
- Castleford (H) beat Widnes (A) (Castleford through to the Grand Final in week four, Widnes play in week three).
- Halifax (A) beat Whitehaven (H) (Halifax will play Widnes in week three's semi final, Whitehaven are eliminated).
- Widnes (H) beat Halifax (A) (Widnes are through to the Grand Final in week four, Halifax are eliminated).
- Castleford Tigers 42 - 10 Widnes Vikings. Match played at neutral venue Headingley Stadium.
Castleford Tigers win promotion to the Super League 2008.