"Rump Shaker" is built on a saxophone sample from the 1972 song "Darkest Light" by Lafayette Afro Rock Band and a drum sample from "Midnight Theme" by Manzel. Other samples include "Scratchin'" by the Magic Disco Machine and "Blues and Pants" by James Brown (the vocal "come on!"). Riley's "Rump Shaker (Teddy 2)" remix adds a bass and piano sample from "Blind Alley" by The Emotions. Additionally, Teddy Riley's verse includes an allusion to the 1982 song "I Like It" by DeBarge, with the lines, "I like the way you comb you hair, I like the stylish clothes you wear, it's just the little things you do...".
The song opens with Teddy Riley chanting the chorus "All I wanna do is zooma-zoom-zoom-zoom in a poom-poom." Subsequent verses are rapped by Aqil Davidson, Teddy Riley, and Markell Riley. Teddy Riley's verse is notable for being written by his young protégéPharrell Williams, later to achieve fame as a member of The Neptunes and a solo artist. It was rumored that Pharrell, along with fellow future-Neptune Chad Hugo, contributed additional production work, but producer Ty Fyffe stated in a 2011 interview that he and Teddy Riley alone produced the song and that Pharrell's only contribution was lyrical. 1
On a Season 4 skit from In Living Color called "Ross Perot Inaugural Interruption", former U.S. Presidential candidate Ross Perot, portrayed by Jim Carrey, buys up the airtime of every single television channel with programming featuring himself. This is done as a ploy to block the inauguration of then U.S. President-elect Bill Clinton under the belief that the inability to broadcast the inauguration prevents it from even happening. One of the programs features Perot being a D.J. working the turntable system while "Rump Shaker" is playing, including him uttering the chorus to the song.
In the "Three Daves" skit from Chappelle's Show, Dave Chappelle (playing himself in the early 1990s) asks a girl why she thinks that all he wants to do is "zooma-zoom" in her "boom-boom".
The song is played in the background of a scene from the 2005 comedy film The 40-Year-Old Virgin. Romany Malco's character, Jay, gyrates to the song at his electronics store workplace while loudly pledging to his virgin co-worker Andy Stitzer, played by Steve Carell, that he will lose his virginity during an upcoming weekend full of endless sex with numerous women through Jay's help.
The song was also referenced in the season 4 episode of American comedy-drama Psych entitled "Death is in the Air" as Shawn's ringtone, heard 3 times throughout the whole episode.
The song was featured in a scene from the TV series Lost in the Season 3 episode, "Exposé".
The song's chorus was used, although with somewhat different lyrics, in the 2008 M.I.A. single "Paper Planes". M.I.A. also incorporates a variation of the chorus "All I wanna do is zoom-zoom-zoom-zoom and boom-boom-boom" in another 2007 song "Hit That".
Darnell and Joy sing the song in an episode of the television series My Name Is Earl