SERCA resides in the sarcoplasmic reticulum (SR) within muscle cells. It is a Ca2+ ATPase that transfers Ca2+ from the cytosol of the cell to the lumen of the SR at the expense of ATP hydrolysis during muscle relaxation.
There are 3 major domains on the cytoplasmic face of SERCA: the phosphorylation and nucleotide-binding domains, which form the catalytic site, and the actuator domain, which is involved in the transmission of major conformational changes.
SERCA is normally inhibited by the defective phosphorylation of protein phospholamban, with which it is closely associated. Another protein, calsequestrin, binds calcium within the SR and helps to reduce the concentration of free calcium within the SR, which assists SERCA so that it does not have to pump against such a high concentration gradient. The SR has a much higher concentration of Ca2+ (10,000x) inside when compared to the intracellular Ca2+ concentration.
The rate at which SERCA moves Ca2+ across the SR membrane can be controlled by phospholamban (PLB/PLN) under β-adrenergic stimulation. Increased β-adrenergic stimulation reduces association between SERCA and PLB by phosphorylation of PLB. When PLB is associated with SERCA, the rate of Ca2+ movement is reduced, upon dissociation of PLB Ca2+ movement increases.
There are 3 major paralogs, SERCA1-3, which are expressed at various levels in different cell types.
There are additional post-translational isoforms of both SERCA2 and SERCA3, which serve to introduce the possibility of cell-type-specific Ca2+-reuptake responses as well as increasing the overall complexity of the Ca2+ signaling mechanism.
- de Meis L, Oliveira GM, Arruda AP, Santos R, Costa RM, Benchimol M (2005). "The thermogenic activity of rat brown adipose tissue and rabbit white muscle Ca2+-ATPase". IUBMB Life 57 (4–5): 337–45. doi:10.1080/15216540500092534. PMID 16036618.
- Arruda AP, Nigro M, Oliveira GM, de Meis L (June 2007). "Thermogenic activity of Ca2+-ATPase from skeletal muscle heavy sarcoplasmic reticulum: the role of ryanodine Ca2+ channel". Biochim. Biophys. Acta 1768 (6): 1498–505. doi:10.1016/j.bbamem.2007.03.016. PMID 17466935.
- Sarcoplasmic Reticulum Calcium-Transporting ATPases at the US National Library of Medicine Medical Subject Headings (MeSH)
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