SF3A1

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Splicing factor 3a, subunit 1, 120kDa
Protein SF3A1 PDB 1we7.png
PDB rendering based on 1we7.
Available structures
PDB Ortholog search: PDBe, RCSB
Identifiers
Symbols SF3A1 ; PRP21; PRPF21; SAP114; SF3A120
External IDs OMIM605595 MGI1914715 HomoloGene4294 GeneCards: SF3A1 Gene
RNA expression pattern
PBB GE SF3A1 201356 at tn.png
PBB GE SF3A1 201357 s at tn.png
PBB GE SF3A1 216457 s at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 10291 67465
Ensembl ENSG00000099995 ENSMUSG00000002129
UniProt Q15459 Q8K4Z5
RefSeq (mRNA) NM_001005409 NM_026175
RefSeq (protein) NP_001005409 NP_080451
Location (UCSC) Chr 22:
30.73 – 30.75 Mb
Chr 11:
4.16 – 4.18 Mb
PubMed search [1] [2]

Splicing factor 3 subunit 1 is a protein that in humans is encoded by the SF3A1 gene.[1][2]

This gene encodes subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 1 belongs to the SURP protein family; named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. Subunit 1 has tandemly repeated SURP motifs in its amino-terminal half while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated subunit 1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3 while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs. Alternative splicing results in multiple transcript variants encoding different isoforms.[2]

Interactions[edit]

SF3A1 has been shown to interact with SF3A3[3][4] and CDC5L.[5]

References[edit]

  1. ^ Kramer A, Mulhauser F, Wersig C, Groning K, Bilbe G (January 1996). "Mammalian splicing factor SF3a120 represents a new member of the SURP family of proteins and is homologous to the essential splicing factor PRP21p of Saccharomyces cerevisiae". RNA 1 (3): 260–72. PMC 1369079. PMID 7489498. 
  2. ^ a b "Entrez Gene: SF3A1 splicing factor 3a, subunit 1, 120kDa". 
  3. ^ Nesic, D; Krämer A (October 2001). "Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation". Mol. Cell. Biol. (United States) 21 (19): 6406–17. doi:10.1128/MCB.21.19.6406-6417.2001. ISSN 0270-7306. PMC 99788. PMID 11533230. 
  4. ^ Chiara, M D; Champion-Arnaud P, Buvoli M, Nadal-Ginard B, Reed R (July 1994). "Specific protein-protein interactions between the essential mammalian spliceosome-associated proteins SAP 61 and SAP 114". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 91 (14): 6403–7. doi:10.1073/pnas.91.14.6403. ISSN 0027-8424. PMC 44210. PMID 8022796. 
  5. ^ Ajuh, P; Kuster B, Panov K, Zomerdijk J C, Mann M, Lamond A I (Dec 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". EMBO J. (ENGLAND) 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. ISSN 0261-4189. PMC 305846. PMID 11101529. 

Further reading[edit]

External links[edit]