SH3RF1

SH3 domain containing ring finger 1
Identifiers
Symbols	SH3RF1; POSH; RNF142; SH3MD2
External IDs	MGI: 1913066 HomoloGene: 10988 GeneCards: SH3RF1 Gene
Gene Ontology Molecular function • MAP-kinase scaffold activity • protein binding • zinc ion binding • ligase activity • metal ion binding Cellular component • cytoplasm • Golgi apparatus • cytosol • lamellipodium • perinuclear region of cytoplasm Biological process • induction of apoptosis • negative regulation of apoptotic process • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process • regulation of JNK cascade • negative regulation of extrinsic apoptotic signaling pathway Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	57630	59009
Ensembl	ENSG00000154447	ENSMUSG00000031642
UniProt	Q7Z6J0	Q69ZI1
RefSeq (mRNA)	NM_020870.3	NM_021506.2
RefSeq (protein)	NP_065921.2	NP_067481.2
Location (UCSC)	Chr 4: 170.02 – 170.19 Mb	Chr 8: 63.7 – 63.87 Mb
PubMed search	[1]	[2]

Putative E3 ubiquitin-protein ligase SH3RF1 is an enzyme that in humans is encoded by the SH3RF1 gene.

This gene encodes a protein containing an N-terminus RING-finger, four SH3 domains, and a region implicated in binding of the Rho GTPase Rac. Via the RING-finger, the encoded protein has been shown to function as a ubiquitin-protein ligase involved in protein sorting at the trans-Golgi network. The encoded protein may also act as a scaffold for the c-Jun N-terminal kinase signaling pathway, facilitating the formation of a functional signaling module.

Interactions

SH3RF1 has been shown to interact with AKT2 and MAP3K11.

Further reading

• Lyons TR, Thorburn J, Ryan PW et al (2007). "Regulation of the Pro-apoptotic scaffolding protein POSH by Akt". J. Biol. Chem. 282 (30): 21987–97. doi:10.1074/jbc.+M704321200. PMID 17535800. 
• Kimura K, Wakamatsu A, Suzuki Y et al (2006). "Diversification of transcriptional modulation: Large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1356129. 
• Alroy I, Tuvia S, Greener T et al (2005). "The trans-Golgi network-associated human ubiquitin-protein ligase POSH is essential for HIV type 1 production". Proc. Natl. Acad. Sci. U.S.A. 102 (5): 1478–83. doi:10.1073/pnas.0408717102. PMC 545085. PMID 15659549. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=545085. 
• Ota T, Suzuki Y, Nishikawa T et al (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039. 
• Figueroa C, Tarras S, Taylor J, Vojtek AB (2004). "Akt2 negatively regulates assembly of the POSH-MLK-JNK signaling complex". J. Biol. Chem. 278 (48): 47922–7. doi:10.1074/jbc.M307357200. PMID 14504284. 
• Strausberg RL, Feingold EA, Grouse LH et al (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Nakayama M, Kikuno R, Ohara O (2003). "Protein–Protein Interactions Between Large Proteins: Two-Hybrid Screening Using a Functionally Classified Library Composed of Long cDNAs". Genome Res. 12 (11): 1773–84. doi:10.1101/gr.406902. PMC 187542. PMID 12421765. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=187542. 
• Nagase T, Kikuno R, Ishikawa K et al (2000). "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (2): 143–50. doi:10.1093/dnares/7.2.143. PMID 10819331.