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S-phase kinase-associated protein 1
Protein SKP1A PDB 1fs1.png
PDB rendering based on 1fs1.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols SKP1 ; EMC19; OCP-II; OCP2; SKP1A; TCEB1L; p19A
External IDs OMIM601434 MGI103575 HomoloGene38775 GeneCards: SKP1 Gene
EC number
RNA expression pattern
PBB GE SKP1A 200718 s at tn.png
PBB GE SKP1A 207974 s at tn.png
PBB GE SKP1A 200711 s at tn.png
More reference expression data
Species Human Mouse
Entrez 6500 21402
Ensembl ENSG00000113558 ENSMUSG00000036309
UniProt P63208 Q9WTX5
RefSeq (mRNA) NM_006930 NM_011543
RefSeq (protein) NP_008861 NP_035673
Location (UCSC) Chr 5:
133.48 – 133.51 Mb
Chr 11:
52.23 – 52.25 Mb
PubMed search [1] [2]

S-phase kinase-associated protein 1 is an enzyme that in humans is encoded by the SKP1 gene.[1][2][3]

This gene encodes a protein that is a member of the SCF ubiquitin ligase protein complex. It binds to F-box proteins (proteins containing an F-box motif), such as cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin dependent proteolysis.[4] The encoded protein also collaborates with a network of proteins to control beta-catenin levels and affects the activity level of beta-catenin dependent TCF transcription factors. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.


SKP1A has been shown to interact with:


  1. ^ Zhang H, Kobayashi R, Galaktionov K, Beach D (October 1995). "p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase". Cell 82 (6): 915–25. doi:10.1016/0092-8674(95)90271-6. PMID 7553852. 
  2. ^ Demetrick DJ, Zhang H, Beach DH (July 1996). "Chromosomal mapping of the genes for the human CDK2/cyclin A-associated proteins p19 (SKP1A and SKP1B) and p45 (SKP2)". Cytogenet Cell Genet 73 (1-2): 104–7. doi:10.1159/000134318. PMID 8646875. 
  3. ^ "Entrez Gene: SKP1A S-phase kinase-associated protein 1A (p19A)". 
  4. ^ Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper JW et al. (1996). "SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box.". Cell 86 (2): 263–74. doi:10.1016/S0092-8674(00)80098-7. PMID 8706131. 
  5. ^ a b c d e f g Cenciarelli C, Chiaur DS, Guardavaccaro D, Parks W, Vidal M, Pagano M (October 1999). "Identification of a family of human F-box proteins". Curr. Biol. 9 (20): 1177–9. doi:10.1016/S0960-9822(00)80020-2. PMID 10531035. 
  6. ^ a b c Min KW, Hwang JW, Lee JS, Park Y, Tamura TA, Yoon JB (May 2003). "TIP120A associates with cullins and modulates ubiquitin ligase activity". J. Biol. Chem. 278 (18): 15905–10. doi:10.1074/jbc.M213070200. PMID 12609982. 
  7. ^ a b Strack P, Caligiuri M, Pelletier M, Boisclair M, Theodoras A, Beer-Romero P et al. (July 2000). "SCF(beta-TRCP) and phosphorylation dependent ubiquitinationof I kappa B alpha catalyzed by Ubc3 and Ubc4". Oncogene 19 (31): 3529–36. doi:10.1038/sj.onc.1203647. PMID 10918611. 
  8. ^ Suzuki H, Chiba T, Suzuki T, Fujita T, Ikenoue T, Omata M et al. (January 2000). "Homodimer of two F-box proteins betaTrCP1 or betaTrCP2 binds to IkappaBalpha for signal-dependent ubiquitination". J. Biol. Chem. 275 (4): 2877–84. doi:10.1074/jbc.275.4.2877. PMID 10644755. 
  9. ^ Margottin F, Bour SP, Durand H, Selig L, Benichou S, Richard V et al. (March 1998). "A novel human WD protein, h-beta TrCp, that interacts with HIV-1 Vpu connects CD4 to the ER degradation pathway through an F-box motif". Mol. Cell 1 (4): 565–74. doi:10.1016/s1097-2765(00)80056-8. PMID 9660940. 
  10. ^ Matsuzawa SI, Reed JC (May 2001). "Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses". Mol. Cell 7 (5): 915–26. doi:10.1016/s1097-2765(01)00242-8. PMID 11389839. 
  11. ^ Ayad NG, Rankin S, Murakami M, Jebanathirajah J, Gygi S, Kirschner MW (April 2003). "Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC". Cell 113 (1): 101–13. doi:10.1016/s0092-8674(03)00232-0. PMID 12679038. 
  12. ^ Kiernan RE, Emiliani S, Nakayama K, Castro A, Labbé JC, Lorca T et al. (Dec 2001). "Interaction between cyclin T1 and SCF(SKP2) targets CDK9 for ubiquitination and degradation by the proteasome". Mol. Cell. Biol. 21 (23): 7956–70. doi:10.1128/MCB.21.23.7956-7970.2001. PMC 99964. PMID 11689688. 
  13. ^ a b Staropoli JF, McDermott C, Martinat C, Schulman B, Demireva E, Abeliovich A (March 2003). "Parkin is a component of an SCF-like ubiquitin ligase complex and protects postmitotic neurons from kainate excitotoxicity". Neuron 37 (5): 735–49. doi:10.1016/s0896-6273(03)00084-9. PMID 12628165. 
  14. ^ Lisztwan J, Marti A, Sutterlüty H, Gstaiger M, Wirbelauer C, Krek W (January 1998). "Association of human CUL-1 and ubiquitin-conjugating enzyme CDC34 with the F-box protein p45(SKP2): evidence for evolutionary conservation in the subunit composition of the CDC34-SCF pathway". EMBO J. 17 (2): 368–83. doi:10.1093/emboj/17.2.368. PMC 1170388. PMID 9430629. 
  15. ^ Zheng N, Schulman BA, Song L, Miller JJ, Jeffrey PD, Wang P et al. (April 2002). "Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex". Nature 416 (6882): 703–9. doi:10.1038/416703a. PMID 11961546. 
  16. ^ Ohta T, Michel JJ, Schottelius AJ, Xiong Y (April 1999). "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity". Mol. Cell 3 (4): 535–41. doi:10.1016/s1097-2765(00)80482-7. PMID 10230407. 
  17. ^ Ewing RM, Chu P, Elisma F, Li H, Taylor P, Climie S et al. "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3: 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. 
  18. ^ Wu G, Lyapina S, Das I, Li J, Gurney M, Pauley A et al. (November 2001). "SEL-10 is an inhibitor of notch signaling that targets notch for ubiquitin-mediated protein degradation". Mol. Cell. Biol. 21 (21): 7403–15. doi:10.1128/MCB.21.21.7403-7415.2001. PMC 99913. PMID 11585921. 
  19. ^ Ng RW, Arooz T, Yam CH, Chan IW, Lau AW, Poon RY (November 1998). "Characterization of the cullin and F-box protein partner Skp1". FEBS Lett. 438 (3): 183–9. doi:10.1016/s0014-5793(98)01299-x. PMID 9827542. 
  20. ^ Schulman BA, Carrano AC, Jeffrey PD, Bowen Z, Kinnucan ER, Finnin MS et al. (November 2000). "Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex". Nature 408 (6810): 381–6. doi:10.1038/35042620. PMID 11099048. 
  21. ^ Marti A, Wirbelauer C, Scheffner M, Krek W (May 1999). "Interaction between ubiquitin-protein ligase SCFSKP2 and E2F-1 underlies the regulation of E2F-1 degradation". Nat. Cell Biol. 1 (1): 14–9. doi:10.1038/8984. PMID 10559858. 

Further reading[edit]