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S-phase kinase-associated protein 1
Protein SKP1A PDB 1fs1.png
PDB rendering based on 1fs1.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols SKP1 ; EMC19; OCP-II; OCP2; SKP1A; TCEB1L; p19A
External IDs OMIM601434 MGI103575 HomoloGene38775 GeneCards: SKP1 Gene
EC number
RNA expression pattern
PBB GE SKP1A 200718 s at tn.png
PBB GE SKP1A 207974 s at tn.png
PBB GE SKP1A 200711 s at tn.png
More reference expression data
Species Human Mouse
Entrez 6500 21402
Ensembl ENSG00000113558 ENSMUSG00000036309
UniProt P63208 Q9WTX5
RefSeq (mRNA) NM_006930 NM_011543
RefSeq (protein) NP_008861 NP_035673
Location (UCSC) Chr 5:
133.48 – 133.51 Mb
Chr 11:
52.23 – 52.25 Mb
PubMed search [1] [2]

S-phase kinase-associated protein 1 is an enzyme that in humans is encoded by the SKP1 gene.[1][2][3]

This gene encodes a protein that is a member of the SCF ubiquitin ligase protein complex. It binds to F-box proteins (proteins containing an F-box motif), such as cyclin F, S-phase kinase-associated protein 2, and other regulatory proteins involved in ubiquitin dependent proteolysis.[4] The encoded protein also collaborates with a network of proteins to control beta-catenin levels and affects the activity level of beta-catenin dependent TCF transcription factors. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.


SKP1A has been shown to interact with FBXL3,[5] FBXO5,[5] FBXO4,[5][6] FBXO7,[5] SKP2,[5][7][8][9][10][11] CDK9,[12] CUL1,[7][13][14][15][16] CDCA3,[17] CACYBP,[18] BTRC,[5][7][8][19][20] FBXW2[5] and FBXW7.[13][21]


  1. ^ Zhang H, Kobayashi R, Galaktionov K, Beach D (October 1995). "p19Skp1 and p45Skp2 are essential elements of the cyclin A-CDK2 S phase kinase". Cell 82 (6): 915–25. doi:10.1016/0092-8674(95)90271-6. PMID 7553852. 
  2. ^ Demetrick DJ, Zhang H, Beach DH (July 1996). "Chromosomal mapping of the genes for the human CDK2/cyclin A-associated proteins p19 (SKP1A and SKP1B) and p45 (SKP2)". Cytogenet Cell Genet 73 (1-2): 104–7. doi:10.1159/000134318. PMID 8646875. 
  3. ^ "Entrez Gene: SKP1A S-phase kinase-associated protein 1A (p19A)". 
  4. ^ Bai et al.; Sen, P; Hofmann, K; Ma, L; Goebl, M; Harper, JW; Elledge, SJ (1996). "SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box.". Cell 86 (2): 263–74. doi:10.1016/S0092-8674(00)80098-7. PMID 8706131. 
  5. ^ a b c d e f g Cenciarelli, C; Chiaur D S; Guardavaccaro D; Parks W; Vidal M; Pagano M (October 1999). "Identification of a family of human F-box proteins". Curr. Biol. (ENGLAND) 9 (20): 1177–9. doi:10.1016/S0960-9822(00)80020-2. ISSN 0960-9822. PMID 10531035. 
  6. ^ Ewing, Rob M; Chu Peter, Elisma Fred, Li Hongyan, Taylor Paul, Climie Shane, McBroom-Cerajewski Linda, Robinson Mark D, O'Connor Liam, Li Michael, Taylor Rod, Dharsee Moyez, Ho Yuen, Heilbut Adrian, Moore Lynda, Zhang Shudong, Ornatsky Olga, Bukhman Yury V, Ethier Martin, Sheng Yinglun, Vasilescu Julian, Abu-Farha Mohamed, Lambert Jean-Philippe, Duewel Henry S, Stewart Ian I, Kuehl Bonnie, Hogue Kelly, Colwill Karen, Gladwish Katharine, Muskat Brenda, Kinach Robert, Adams Sally-Lin, Moran Michael F, Morin Gregg B, Topaloglou Thodoros, Figeys Daniel (2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. (England) 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. 
  7. ^ a b c Min, Kyoeng-Woo; Hwang Ji-Won; Lee Jong-Sik; Park Yoon; Tamura Taka-aki; Yoon Jong-Bok (May 2003). "TIP120A associates with cullins and modulates ubiquitin ligase activity". J. Biol. Chem. (United States) 278 (18): 15905–10. doi:10.1074/jbc.M213070200. ISSN 0021-9258. PMID 12609982. 
  8. ^ a b Strack, P; Caligiuri M, Pelletier M, Boisclair M, Theodoras A, Beer-Romero P, Glass S, Parsons T, Copeland R A, Auger K R, Benfield P, Brizuela L, Rolfe M (July 2000). "SCF(beta-TRCP) and phosphorylation dependent ubiquitinationof I kappa B alpha catalyzed by Ubc3 and Ubc4". Oncogene (ENGLAND) 19 (31): 3529–36. doi:10.1038/sj.onc.1203647. ISSN 0950-9232. PMID 10918611. 
  9. ^ Ng, R W; Arooz T; Yam C H; Chan I W; Lau A W; Poon R Y (November 1998). "Characterization of the cullin and F-box protein partner Skp1". FEBS Lett. (NETHERLANDS) 438 (3): 183–9. doi:10.1016/S0014-5793(98)01299-X. ISSN 0014-5793. PMID 9827542. 
  10. ^ Schulman, B A; Carrano A C; Jeffrey P D; Bowen Z; Kinnucan E R; Finnin M S; Elledge S J; Harper J W; Pagano M; Pavletich N P (November 2000). "Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex". Nature (ENGLAND) 408 (6810): 381–6. doi:10.1038/35042620. ISSN 0028-0836. PMID 11099048. 
  11. ^ Marti, A; Wirbelauer C; Scheffner M; Krek W (May 1999). "Interaction between ubiquitin-protein ligase SCFSKP2 and E2F-1 underlies the regulation of E2F-1 degradation". Nat. Cell Biol. (ENGLAND) 1 (1): 14–9. doi:10.1038/8984. ISSN 1465-7392. PMID 10559858. 
  12. ^ Kiernan, R E; Emiliani S; Nakayama K; Castro A; Labbé J C; Lorca T; Nakayama Ki K; Benkirane M (Dec 2001). "Interaction between cyclin T1 and SCF(SKP2) targets CDK9 for ubiquitination and degradation by the proteasome". Mol. Cell. Biol. (United States) 21 (23): 7956–70. doi:10.1128/MCB.21.23.7956-7970.2001. ISSN 0270-7306. PMC 99964. PMID 11689688. 
  13. ^ a b Staropoli, John F; McDermott Caroline; Martinat Cécile; Schulman Brenda; Demireva Elena; Abeliovich Asa (March 2003). "Parkin is a component of an SCF-like ubiquitin ligase complex and protects postmitotic neurons from kainate excitotoxicity". Neuron (United States) 37 (5): 735–49. doi:10.1016/S0896-6273(03)00084-9. ISSN 0896-6273. PMID 12628165. 
  14. ^ Lisztwan, J; Marti A; Sutterlüty H; Gstaiger M; Wirbelauer C; Krek W (January 1998). "Association of human CUL-1 and ubiquitin-conjugating enzyme CDC34 with the F-box protein p45(SKP2): evidence for evolutionary conservation in the subunit composition of the CDC34-SCF pathway". EMBO J. (ENGLAND) 17 (2): 368–83. doi:10.1093/emboj/17.2.368. ISSN 0261-4189. PMC 1170388. PMID 9430629. 
  15. ^ Zheng, Ning; Schulman Brenda A, Song Langzhou, Miller Julie J, Jeffrey Philip D, Wang Ping, Chu Claire, Koepp Deanna M, Elledge Stephen J, Pagano Michele, Conaway Ronald C, Conaway Joan W, Harper J Wade, Pavletich Nikola P (April 2002). "Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex". Nature (England) 416 (6882): 703–9. doi:10.1038/416703a. ISSN 0028-0836. PMID 11961546. 
  16. ^ Ohta, T; Michel J J; Schottelius A J; Xiong Y (April 1999). "ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity". Mol. Cell (UNITED STATES) 3 (4): 535–41. doi:10.1016/S1097-2765(00)80482-7. ISSN 1097-2765. PMID 10230407. 
  17. ^ Ayad, Nagi G; Rankin Susannah; Murakami Monica; Jebanathirajah Judith; Gygi Steven; Kirschner Marc W (April 2003). "Tome-1, a trigger of mitotic entry, is degraded during G1 via the APC". Cell (United States) 113 (1): 101–13. doi:10.1016/S0092-8674(03)00232-0. ISSN 0092-8674. PMID 12679038. 
  18. ^ Matsuzawa, S I; Reed J C (May 2001). "Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses". Mol. Cell (United States) 7 (5): 915–26. doi:10.1016/S1097-2765(01)00242-8. ISSN 1097-2765. PMID 11389839. 
  19. ^ Suzuki, H; Chiba T; Suzuki T; Fujita T; Ikenoue T; Omata M; Furuichi K; Shikama H; Tanaka K (January 2000). "Homodimer of two F-box proteins betaTrCP1 or betaTrCP2 binds to IkappaBalpha for signal-dependent ubiquitination". J. Biol. Chem. (UNITED STATES) 275 (4): 2877–84. doi:10.1074/jbc.275.4.2877. ISSN 0021-9258. PMID 10644755. 
  20. ^ Margottin, F; Bour S P; Durand H; Selig L; Benichou S; Richard V; Thomas D; Strebel K; Benarous R (March 1998). "A novel human WD protein, h-beta TrCp, that interacts with HIV-1 Vpu connects CD4 to the ER degradation pathway through an F-box motif". Mol. Cell (UNITED STATES) 1 (4): 565–74. doi:10.1016/S1097-2765(00)80056-8. ISSN 1097-2765. PMID 9660940. 
  21. ^ Wu, G; Lyapina S; Das I; Li J; Gurney M; Pauley A; Chui I; Deshaies R J; Kitajewski J (November 2001). "SEL-10 is an inhibitor of notch signaling that targets notch for ubiquitin-mediated protein degradation". Mol. Cell. Biol. (United States) 21 (21): 7403–15. doi:10.1128/MCB.21.21.7403-7415.2001. ISSN 0270-7306. PMC 99913. PMID 11585921. 

Further reading[edit]