Structural maintenance of chromosomes 3, also known as SMC3, is a human gene. This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein.
Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. Twenty two tests were carried out on mutant mice and six significant abnormalities were observed. No homozygousmutant embryos were identified during gestation, and thus none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice. Females had a higher than normal incidence of pre-wean death in their offspring, and also had a decreased body weight. Males heterozygotes displayed a shortened, upturned snout.
^ abLee, Jibak; Iwai Toshiharu, Yokota Takehiro, Yamashita Masakane (July 2003). "Temporally and spatially selective loss of Rec8 protein from meiotic chromosomes during mammalian meiosis". J. Cell. Sci. (England) 116 (Pt 13): 2781–90. doi:10.1242/jcs.00495. ISSN0021-9533. PMID12759374.Cite uses deprecated parameters (help)
^ abGupta, K; Anand G, Yin X, Grove L, Prochownik E V (March 1998). "Mmip1: a novel leucine zipper protein that reverses the suppressive effects of Mad family members on c-myc". Oncogene (ENGLAND) 16 (9): 1149–59. doi:10.1038/sj.onc.1201634. ISSN0950-9232. PMID9528857.Cite uses deprecated parameters (help)
^Shimizu, K; Shirataki H, Honda T, Minami S, Takai Y (March 1998). "Complex formation of SMAP/KAP3, a KIF3A/B ATPase motor-associated protein, with a human chromosome-associated polypeptide". J. Biol. Chem. (UNITED STATES) 273 (12): 6591–4. doi:10.1074/jbc.273.12.6591. ISSN0021-9258. PMID9506951.Cite uses deprecated parameters (help)
^Gregson, H C; Schmiesing J A, Kim J S, Kobayashi T, Zhou S, Yokomori K (Dec 2001). "A potential role for human cohesin in mitotic spindle aster assembly". J. Biol. Chem. (United States) 276 (50): 47575–82. doi:10.1074/jbc.M103364200. ISSN0021-9258. PMID11590136.Cite uses deprecated parameters (help)
Shimizu K, Shirataki H, Honda T, et al. (1998). "Complex formation of SMAP/KAP3, a KIF3A/B ATPase motor-associated protein, with a human chromosome-associated polypeptide". J. Biol. Chem.273 (12): 6591–4. doi:10.1074/jbc.273.12.6591. PMID9506951.
Gupta K, Anand G, Yin X, et al. (1998). "Mmip1: a novel leucine zipper protein that reverses the suppressive effects of Mad family members on c-myc". Oncogene16 (9): 1149–59. doi:10.1038/sj.onc.1201634. PMID9528857.
James RD, Schmiesing JA, Peters AH, et al. (2003). "Differential association of SMC1alpha and SMC3 proteins with meiotic chromosomes in wild-type and SPO11-deficient male mice". Chromosome Res.10 (7): 549–60. doi:10.1023/A:1020910601858. PMID12498344.
Ghiselli G, Coffee N, Munnery CE, et al. (2003). "The cohesin SMC3 is a target the for beta-catenin/TCF4 transactivation pathway". J. Biol. Chem.278 (22): 20259–67. doi:10.1074/jbc.M209511200. PMID12651860.
Lee J, Iwai T, Yokota T, Yamashita M (2004). "Temporally and spatially selective loss of Rec8 protein from meiotic chromosomes during mammalian meiosis". J. Cell. Sci.116 (Pt 13): 2781–90. doi:10.1242/jcs.00495. PMID12759374.
Rankin S, Ayad NG, Kirschner MW (2005). "Sororin, a substrate of the anaphase-promoting complex, is required for sister chromatid cohesion in vertebrates". Mol. Cell18 (2): 185–200. doi:10.1016/j.molcel.2005.03.017. PMID15837422.