SNAP29

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Synaptosomal-associated protein, 29kDa
Identifiers
Symbols SNAP29 ; CEDNIK; SNAP-29
External IDs OMIM604202 MGI1914724 HomoloGene3512 GeneCards: SNAP29 Gene
RNA expression pattern
PBB GE SNAP29 218327 s at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 9342 67474
Ensembl ENSG00000099940 ENSMUSG00000022765
UniProt O95721 Q9ERB0
RefSeq (mRNA) NM_004782 NM_023348
RefSeq (protein) NP_004773 NP_075837
Location (UCSC) Chr 22:
21.21 – 21.25 Mb
Chr 16:
17.41 – 17.43 Mb
PubMed search [1] [2]

Synaptosomal-associated protein 29 is a protein that in humans is encoded by the SNAP29 gene.[1][2][3] This gene, a member of the SNAP25 gene family, encodes a protein involved in multiple membrane trafficking steps. Two other members of this gene family, SNAP23 and SNAP25, encode proteins that bind a syntaxin protein and mediate synaptic vesicle membrane docking and fusion to the plasma membrane. The protein encoded by this gene binds tightly to multiple syntaxins and is localized to intracellular membrane structures rather than to the plasma membrane. While the protein is mostly membrane-bound, a significant fraction of it is found free in the cytoplasm. Use of multiple polyadenylation sites has been noted for this gene.[3]

Model organisms[edit]

Model organisms have been used in the study of SNAP29 function. A conditional knockout mouse line, called Snap29tm1a(EUCOMM)Wtsi[8][9] was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.[10][11][12]

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion.[6][13] Twenty five tests were carried out on mutant mice and two significant abnormalities were observed.[6] No homozygous mutant embryos were identified during gestation, and therefore none survived until weaning. The remaining tests were carried out on heterozygous mutant adult mice; no significant abnormalities were observed in these animals.[6]

Interactions[edit]

SNAP29 has been shown to interact with Syntaxin 3[1] and EHD1.[14]

References[edit]

  1. ^ a b Steegmaier M, Yang B, Yoo JS, Huang B, Shen M, Yu S, Luo Y, Scheller RH (January 1999). "Three novel proteins of the syntaxin/SNAP-25 family". J Biol Chem 273 (51): 34171–9. doi:10.1074/jbc.273.51.34171. PMID 9852078. 
  2. ^ Dunham I, Shimizu N, Roe BA, Chissoe S, Hunt AR, Collins JE, Bruskiewich R, Beare DM, Clamp M (Dec 1999). "The DNA sequence of human chromosome 22". Nature 402 (6761): 489–95. doi:10.1038/990031. PMID 10591208. 
  3. ^ a b "Entrez Gene: SNAP29 synaptosomal-associated protein, 29kDa". 
  4. ^ "Salmonella infection data for Snap29". Wellcome Trust Sanger Institute. 
  5. ^ "Citrobacter infection data for Snap29". Wellcome Trust Sanger Institute. 
  6. ^ a b c d Gerdin AK (2010). "The Sanger Mouse Genetics Programme: High throughput characterisation of knockout mice". Acta Ophthalmologica 88: 925–7. doi:10.1111/j.1755-3768.2010.4142.x. 
  7. ^ Mouse Resources Portal, Wellcome Trust Sanger Institute.
  8. ^ "International Knockout Mouse Consortium". 
  9. ^ "Mouse Genome Informatics". 
  10. ^ Skarnes, W. C.; Rosen, B.; West, A. P.; Koutsourakis, M.; Bushell, W.; Iyer, V.; Mujica, A. O.; Thomas, M.; Harrow, J.; Cox, T.; Jackson, D.; Severin, J.; Biggs, P.; Fu, J.; Nefedov, M.; De Jong, P. J.; Stewart, A. F.; Bradley, A. (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337–342. doi:10.1038/nature10163. PMC 3572410. PMID 21677750.  edit
  11. ^ Dolgin E (2011). "Mouse library set to be knockout". Nature 474 (7351): 262–3. doi:10.1038/474262a. PMID 21677718. 
  12. ^ Collins FS, Rossant J, Wurst W (2007). "A Mouse for All Reasons". Cell 128 (1): 9–13. doi:10.1016/j.cell.2006.12.018. PMID 17218247. 
  13. ^ van der Weyden L, White JK, Adams DJ, Logan DW (2011). "The mouse genetics toolkit: revealing function and mechanism.". Genome Biol 12 (6): 224. doi:10.1186/gb-2011-12-6-224. PMC 3218837. PMID 21722353. 
  14. ^ Rotem-Yehudar, R; Galperin E, Horowitz M (August 2001). "Association of insulin-like growth factor 1 receptor with EHD1 and SNAP29". J. Biol. Chem. (United States) 276 (35): 33054–60. doi:10.1074/jbc.M009913200. ISSN 0021-9258. PMID 11423532. 

Further reading[edit]