SNARE (protein)

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Molecular machinery driving vesicle fusion in neuromediator release. The core SNARE complex is formed by four α-helices contributed by synaptobrevin, syntaxin and SNAP-25, synaptotagmin serves as a calcium sensor and regulates intimately the SNARE zipping

SNARE proteins (an acronym derived from "SNAP (Soluble NSF Attachment Protein) REceptor") are a large protein superfamily consisting of more than 60 members in yeast and mammalian cells.[1]

The primary role of SNARE proteins is to mediate vesicle fusion, that is, the exocytosis of cellular transport vesicles with the cell membrane at the porosome or with a target compartment (such as a lysosome).

SNAREs can be divided into two categories: vesicle or v-SNAREs, which are incorporated into the membranes of transport vesicles during budding, and target or t-SNAREs, which are located in the membranes of target compartments.

Recent classification however takes account of the structural features of the SNARE proteins and divides them into R-SNAREs and Q-SNAREs.

The best-studied SNAREs are those that mediate docking of synaptic vesicles with the presynaptic membrane. These SNAREs are the targets of the bacterial neurotoxins responsible for botulism and tetanus.

SNAREs are small, abundant and mostly plasma membrane-bound proteins. Although they vary considerably in structure and size, all share a segment in their cytosolic domain called a SNARE motif that consists of 60-70 amino acids that are capable of reversible assembly into tight, four-helix bundles called "trans"-SNARE complexes.

The readily-formed metastable "trans" complexes are composed of three SNAREs: syntaxin 1 and SNAP-25 resident in cell membrane and synaptobrevin (also referred to as vesicle-associated membrane protein or VAMP) anchored in the vesicular membrane.

In neuronal exocytosis, syntaxin and synaptobrevin are anchored in respective membranes by their C-terminal domains, whereas SNAP-25 is tethered to the plasma membrane via several cysteine-linked palmitoyl chains. The core SNARE complex is a four-\alpha-helix bundle, where one \alpha-helix is contributed by syntaxin-1, one \alpha-helix by synaptobrevin and two \alpha-helices are contributed by SNAP-25.

The plasma membrane-resident SNAREs have been shown to be present in distinct microdomains or clusters, the integrity of which is essential for the exocytotic competence of the cell.

SNARE complexes[edit]

Layering of the core SNARE complex. In the center is the zero hydrophilic ionic layer, flanked by hydrophobic leucine-zipper layers.

During membrane fusion, the SNARE proteins involved combine to form a SNARE complex. Depending on the stage of fusion of the host vesicles, these complexes may be referred to differently.

"Trans"-SNARE complexes are protein complexes composed of three SNARE proteins anchored in opposing (or trans) membranes prior to membrane fusion. During fusion, the membranes merge and SNARE proteins involved in complex formation after fusion are then referred to as a "cis"-SNARE complex, because they now reside in a single (or cis) resultant membrane.

R-SNAREs[edit]

R-SNAREs are proteins that contribute an arginine (R) residue in the formation of the zero ionic layer in the assembled core SNARE complex. One particular R-SNARE is synaptobrevin, which is located in the synaptic vesicles.

Q-SNAREs[edit]

Q-SNAREs are proteins that contribute a glutamine (Q) residue in the formation of the zero ionic layer in the assembled core SNARE complex. Q-SNAREs include syntaxin and SNAP-25.

Other components[edit]

The core SNARE complex is a 4-\alpha-helix bundle.[2] Synaptobrevin and syntaxin contribute one \alpha-helix each, while SNAP-25 participates with two \alpha-helices (abbreviated as Sn1 and Sn2). The interacting amino acid residues that zip the SNARE complex can be grouped into layers. Each layer has 4 amino acid residues - one residue per each of the 4 \alpha-helices. In the center of the complex is the zero ionic layer composed of one arginine (R) and three glutamine (Q) residues, and it is flanked by leucine zippering. Layers '-1', '+1' and '+2' at the centre of the complex most closely follow ideal leucine-zipper geometry and aminoacid composition.[3]

The zero ionic layer is composed of R56 from VAMP-2, Q226 from syntaxin-1A, Q53 from Sn1 and Q174 from Sn2, and is completely buried within the leucine-zipper layers. The positively charged guanidino group of the arginine (R) residue interact with the carboxyl groups of each of the three glutamine (Q) residues.

The flanking leucine-zipper layers act as a water-tight seal to shield the ionic interactions from the surrounding solvent. Exposure of the zero ionic layer to the water solvent by breaking the flanking leucine zipper leads to instability of the SNARE complex and is the putative mechanism by which \alpha-SNAP and NSF recycle the SNARE complexes after the completion of synaptic vesicle exocytosis.

Proposed mechanism of membrane fusion[edit]

Assembly of the SNAREs into the "trans" complexes likely bridges the opposing lipid bilayers of membranes belonging to cell and secretory granule, bringing them in proximity and inducing their fusion. The influx of calcium into the cell triggers the completion of the assembly reaction, which is mediated by an interaction between the putative calcium sensor, synaptotagmin, with membrane lipids and/or the partially assembled SNARE complex.

According to the "zipper" hypothesis, the complex assembly starts at the N-terminal parts of SNARE motifs and proceeds towards the C-termini that anchor interacting proteins in membranes. Formation of the "trans"-SNARE complex proceeds through an intermediate complex composed of SNAP-25 and syntaxin-1, which later accommodates synaptobrevin-2 (the quoted syntaxin and synaptobrevin isotypes participate in neuronal neuromediator release).

Based on the stability of the resultant cis-SNARE complex, it has been postulated that energy released during the assembly process serves as a means for overcoming the repulsive forces between the membranes. There are several models that propose explanation of a subsequent step – the formation of stalk and fusion pore, but the exact nature of these processes remains debated. A recent in vitro single-molecule content-mixing study showed that yeast SNARE complex is enough to expand fusion pores.[4]

According to the "clamp" hypothesis, a reversible clamping protein (known as complexin), inhibits synaptic vesicle fusion. When calcium binds to the calcium sensor synaptotagmin, the clamp would then be released. SNARE proteins, and key regulators like synaptotagmin and complexin, act as markers on the cell membrane. Cells expressing such “flipped” synaptic SNARE switches fuse constitutionally. However, when the expression of complexin blocked fusion alone. When calcium was added back, the cell began to exhibit fusion. This suggests that synaptotagmin and complexin may be co-regulators in synaptic vesicle fusion.[5]

However, recent evidence including detailed structural and functional studies have proposed that SNAREs mostly function in accord with the "zipper" model. Nevertheless, it remains unclear whether SNARE assembly between membranes directly leads to the merger of lipid bilayers.[6]

Toxins[edit]

Many neurotoxins directly affect SNARE complexes. Such toxins as the botulinum and tetanus toxins work by targeting the SNARE components. These toxins prevent proper vesicle recycling and result in poor muscle control, spasms, paralysis, and even death.

Specifically, the botulinum toxin attacks the SNAP-25 protein of the SNARE complex. The botulinum toxin degrades and cleaves the SNAP-25 protein, a protein that is required for vesicle fusion that releases neurotransmitters. Botulinum toxin essentially cleaves these SNARE proteins, and in doing so, prevents synaptic vesicles from fusing with the cellular synaptic membrane and releasing their neurotransmitters. The tetanus toxin follows a similar pathway, but instead attacks the protein synaptobrevin on the synaptic vesicle.

These toxins result in acquiring tetanus, a medical condition characterized by a prolonged contraction of skeletal muscle fibers, and botulism, a type of food poisoning that can lead to muscle paralysis including breathing muscles, causing respiratory failure.

Botulinum Neurotoxin (BoNT)[edit]

Botulinum Toxin (BoNT) is one of the most potent toxins to have ever been discovered.[7] It is a proteolytic enzyme that cleaves SNARE proteins in neurons. It's structure is composed of a Heavy Chain (larger peptide subunit) about 100kDas and a Light Chain (smaller peptide subunit) of about 50kDas, which are held together by a disulfide bond. The action of BoNT follows a 4-step mechanism including binding to the neuronal membrane, endocytosis, membrane translocation, and proteolytic cleavage of SNARE proteins.[8]

Target SNARE proteins of Botulinum Neurotoxin (BoNT) and Tetanus Neurotoxin (TeNT) inside the axon terminal. [9]

First, the Heavy Chain of BoNT is used to find its neuronal targets and bind to the gangliosides and membrane proteins of presynaptic neurons. Next, the toxin is then endocytosed into the cell membrane. The Heavy Chain undergoes a conformational change important for translocating the Light Chain into the cytosol of the neuron. Finally, after the Light Chain of BoNT is brought into the cytosol of the targeted neuron, it is released from the Heavy Chain so that it can reach its active cleavage sites on the SNARE proteins.[8] The Light Chain is released from the Heavy Chain by the reduction of the disulfide bond holding the two together. The reduction of this disulfide bond is mediated by the NADPH-thioredoxin reductase-thioredoxin system.[10] The Light Chain of BoNT acts as a metalloprotease on SNARE proteins that is dependent on Zn(II) ions,[11] cleaving them and eliminating their function in exocytosis.

There are 8 known isotypes of BoNT, named BoNT/A - BoNT/H, with different specific cleavage sites on SNARE proteins. SNAP25 is a member of the SNARE protein family located in the membrane of cells. BoNT isotypes A, C, and E target SNAP-25 proteins in neuronal membranes and cleave them. BoNT/C Also targets Syntaxin-1, another SNARE protein located in the membrane of cells, and degenerates these proteins with a similar outcome as with SNAP-25. A third SNARE protein, Synaptobrevin (VAMP), is located on cell vesicles. VAMP2 is targeted and cleaved by BoNT isotypes B, D, F in synaptic neurons.[7]

In each case, Botuliunum Neurotoxins cause functional damage to SNARE proteins. By doing so, this prevents synaptic vesicles from fusing with the cellular synaptic membrane and releasing their neurotransmitters into the synaptic cleft. This results in acquiring botulism, which can lead to muscle paralysis including breathing muscles, causing respiratory failure.

Tetanus Neurotoxin (TeNT)[edit]

The breakdown of responsibilities and mechanisms of the heavy (HC) and light chain (LC) of tetanus neurotoxin: The HC assists in binding of TeNT to both the ganglioside receptor and the final receptor. Once TeNT is in the vesicle in the inhibitory interneuron space the HC assists in translocation of the LC into the cytoplasm. Then the LC, characterized by zinc endopeptidase activity, inhibits neurotransmission by cleavage of synaptobrevin 1.

Tetanus toxin, or TeNT, is composed of a heavy chain (100KDa) and a light chain (50kDa) connected by a disulfide bond. The heavy chain is responsible for neurospecific binding of TeNT to the nerve terminal membrane, endocytosis of the toxin, and translocation of the light chain into the cytosol. The light chain has zinc-dependent endopepdtidase or more specifically matrix metalloproteinase (MMP) activity through which cleaveage of synaptobrevin or VAMP is carried out.[12]

For the light chain of TeNT to be activated one atom of zinc must be bound to every molecule of toxin.[13] When zinc is bound reduction of the disulfide bond will be carried out primarily via the NADPH-thioredoxin reductase-thioredoxin redox system.[14] Then the light chain is free to cleave the Gln76-Phe77 bond of synaptobrevin.[12] Cleavage of synaptobrevin affects the stability of the SNARE core by restricting it from entering the low energy conformation which is the target for NSF binding.[15] This cleavage of synaptobrevin is the final target of TeNT and even in low doses the neurotoxin will inhibit neurotransmitter exocytosis.

References[edit]

  1. ^ Gerald K (2002). "Cell and Molecular Biology (4th edition)". John Wiley & Sons, Inc. 
  2. ^ Sutton RB, Fasshauer D, Jahn R, Brunger AT (1998). "Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 Å resolution". Nature 395 (6700): 347–353. doi:10.1038/26412. PMID 9759724. 
  3. ^ Fasshauer D, Sutton RB, Brunger AT, Jahn R (1998). "Conserved structural features of the synaptic fusion complex: SNARE proteins reclassified as Q- and R-SNAREs". Proceedings of the National Academy of Sciences 95 (26): 15781–15786. doi:10.1073/pnas.95.26.15781. PMC 28121. PMID 9861047. 
  4. ^ Diao J, Su Z, Lu X, Yoon TY, Shin YK, Ha T (2010). "Single-Vesicle Fusion Assay Reveals Munc18-1 Binding to the SNARE Core Is Sufficient for Stimulating Membrane Fusion". ACS Chem Neurosci 1 (3): 168–174. doi:10.1021/cn900034p. PMC 2841011. PMID 20300453. 
  5. ^ Giraudo CG, Eng WS, Melia TJ, Rothman JE (2006). "A Clamping Mechanism Involved in SNARE-Dependent Exocytosis". Science 313 (5787): 676–680. doi:10.1126/science.1129450. PMID 16794037. 
  6. ^ Fasshauer D (2003). "Structural insights into the SNARE mechanism". Biochimica et biophysica acta 1641 (2–3): 87–97. doi:10.1016/S0167-4889(03)00090-9. PMID 12914950. 
  7. ^ a b Peng, Lisheng; Liu, Huisheng; Ruan, Hongyu; Tepp, William H.; Stoothoff, William H.; Brown, Robert H.; Johnson, Eric A.; Yao, Wei-Dong; Zhang, Su-Chun; Dong, Min (12 February 2013). "Cytotoxicity of botulinum neurotoxins reveals a direct role of syntaxin 1 and SNAP-25 in neuron survival". Nature Communications 4: 1472. doi:10.1038/ncomms2462. Retrieved 12 November 2014. 
  8. ^ a b Rossetto, Ornella; Pirazzini, Marco; Balognese, Paolo; Rigoni, Michela; Montecucco, Cesare (2011). "An Update on the Mechanism of Action of Tetanus and Botulinum Neurotoxins". ACTA CHIMICA SLOVENICA 58 (4): 702–707. Retrieved 12 November 2014. 
  9. ^ Barr, John R.; Moura, Hercules; Boyer, Anne E.; Woolfitt, Adrian R.; Kalb, Suzanne R.; Pavlopoulos, Antonis; McWiliams, Lisa G.; Schmidt, Jugen G.; Martinez, Rodolfo A.; Ashley, David L. (October 2005). "Botulinum Neurotoxin Detection and Differentiation by Mass Spectrometry". Emerging Infectious Disease 11 (10): 1578. Retrieved 18 November 2014. 
  10. ^ Pirazzini, Marco; Bordin, Fulvio; Rossetto, Ornella; Shone, Clifford C.; Binz, Thomas; Montecucco, Cesare (January 2013). "The thioredoxin reductase-thioredoxin system is involved in the entry of tetanus and botulinum neurotoxins in the cytosol of nerve terminals". FEBS Letters 587 (2): 150–155. doi:10.1016/j.febslet.2012.11.007. Retrieved 12 November 2014. 
  11. ^ Silvaggi, Nicholas R.; Wilson, David; Tzipori, Saul; Allen, Karen N. (May 2008). "Catalytic Features of the Botulinum Neurotoxin A Light Chain Revealed by High Resolution Structure of an Inhibitory Peptide Complex". Biochemistry 47 (21): 5736–5745. doi:10.1021/bi8001067. Retrieved 18 November 2014. 
  12. ^ a b Schiavo, G; Benfenati, F; Poulain, B; Rossetto, O; Polverino de Laureto, P; DasGupta, BR; Montecucco, C (29 October 1992). "Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin.". Nature 359 (6398): 832–5. PMID 1331807. 
  13. ^ Schiavo, G; Poulain, B; Rossetto, O; Benfenati, F; Tauc, L; Montecucco, C (October 1992). "Tetanus toxin is a zinc protein and its inhibition of neurotransmitter release and protease activity depend on zinc.". The EMBO journal 11 (10): 3577–83. PMID 1396558. 
  14. ^ Pirazzini, M; Bordin, F; Rossetto, O; Shone, CC; Binz, T; Montecucco, C (16 January 2013). "The thioredoxin reductase-thioredoxin system is involved in the entry of tetanus and botulinum neurotoxins in the cytosol of nerve terminals.". FEBS letters 587 (2): 150–5. PMID 23178719. 
  15. ^ Pellegrini, LL; O'Connor, V; Lottspeich, F; Betz, H (2 October 1995). "Clostridial neurotoxins compromise the stability of a low energy SNARE complex mediating NSF activation of synaptic vesicle fusion.". The EMBO journal 14 (19): 4705–13. PMID 7588600. 


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