From Wikipedia, the free encyclopedia
Jump to: navigation, search
Spi-1 proto-oncogene
Protein SPI1 PDB 1pue.png
PDB rendering based on 1pue.
Available structures
PDB Ortholog search: PDBe, RCSB
Symbols SPI1 ; OF; PU.1; SFPI1; SPI-1; SPI-A
External IDs OMIM165170 MGI98282 HomoloGene2346 GeneCards: SPI1 Gene
RNA expression pattern
PBB GE SPI1 205312 at tn.png
More reference expression data
Species Human Mouse
Entrez 6688 20375
Ensembl ENSG00000066336 ENSMUSG00000002111
UniProt P17947 P17433
RefSeq (mRNA) NM_001080547 NM_011355
RefSeq (protein) NP_001074016 NP_035485
Location (UCSC) Chr 11:
47.38 – 47.4 Mb
Chr 2:
91.08 – 91.12 Mb
PubMed search [1] [2]

Transcription factor PU.1 is a protein that in humans is encoded by the SPI1 gene.[1]

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.[2]


SPI1 has been shown to interact with NONO,[3] GATA2,[4] FUS[5] and IRF4.[6][7]

See also[edit]


  1. ^ Ray D, Culine S, Tavitain A, Moreau-Gachelin F (July 1990). "The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors". Oncogene 5 (5): 663–8. PMID 1693183. 
  2. ^ "Entrez Gene: SPI1 spleen focus forming virus (SFFV) proviral integration oncogene spi1". 
  3. ^ Hallier, M; Tavitian A; Moreau-Gachelin F (May 1996). "The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb". J. Biol. Chem. (UNITED STATES) 271 (19): 11177–81. doi:10.1074/jbc.271.19.11177. ISSN 0021-9258. PMID 8626664. 
  4. ^ Zhang, P; Behre G; Pan J; Iwama A; Wara-Aswapati N; Radomska H S; Auron P E; Tenen D G; Sun Z (July 1999). "Negative cross-talk between hematopoietic regulators: GATA proteins repress PU.1". Proc. Natl. Acad. Sci. U.S.A. (UNITED STATES) 96 (15): 8705–10. doi:10.1073/pnas.96.15.8705. ISSN 0027-8424. PMC 17580. PMID 10411939. 
  5. ^ Hallier, M; Lerga A; Barnache S; Tavitian A; Moreau-Gachelin F (February 1998). "The transcription factor Spi-1/PU.1 interacts with the potential splicing factor TLS". J. Biol. Chem. (UNITED STATES) 273 (9): 4838–42. doi:10.1074/jbc.273.9.4838. ISSN 0021-9258. PMID 9478924. 
  6. ^ Brass, A L; Zhu A Q; Singh H (February 1999). "Assembly requirements of PU.1-Pip (IRF-4) activator complexes: inhibiting function in vivo using fused dimers". EMBO J. (ENGLAND) 18 (4): 977–91. doi:10.1093/emboj/18.4.977. ISSN 0261-4189. PMC 1171190. PMID 10022840. 
  7. ^ Escalante, Carlos R; Shen Leyi; Escalante Mary C; Brass Abraham L; Edwards Thomas A; Singh Harinder; Aggarwal Aneel K (July 2002). "Crystallization and characterization of PU.1/IRF-4/DNA ternary complex". J. Struct. Biol. (United States) 139 (1): 55–9. doi:10.1016/S1047-8477(02)00514-2. ISSN 1047-8477. PMID 12372320. 

Further reading[edit]

External links[edit]